Phosphotransferase activity in Clostridium acetobutylicum from acidogenic and solventogenic phases of growth

R. W. Hutkins, E. R. Kashket

Research output: Contribution to journalArticlepeer-review

30 Scopus citations

Abstract

Clostridium acetobutylicum cells, when energized with fructose, transported and phosphorylated the glucose analog 2-deoxyglucose by a phosphoenolpyruvate-dependent phosphotransferase (PT) system. Butanol up to 2% did not inhibit PT activity, although its chaotropic effect on the cell membrane caused cellular phosphoenolpyruvate and the 2-deoxyglucose-6-phosphate to leak out. Cells harvested from the solventogenic phase of batch growth had a significantly lower PT activity than did cells from the acidogenic phase.

Original languageEnglish (US)
Pages (from-to)1121-1123
Number of pages3
JournalApplied and environmental microbiology
Volume51
Issue number5
DOIs
StatePublished - 1986

ASJC Scopus subject areas

  • Biotechnology
  • Food Science
  • Applied Microbiology and Biotechnology
  • Ecology

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