Physical characterization of PBCV virus by sedimentation field flow fractionation

Clement R. Yonker, Karin D. Caldwell, J. Calvin Giddings, James L. Van Etten

Research output: Contribution to journalArticle

23 Scopus citations

Abstract

A virus (PBCV) from the symbiotic Chlorella-like algae of Paramecium bursaria has been characterized by sedimentation field flow fractionation (SdFFF). The method yields effective molecular weight M′ (molecular weight × buoyancy factor) with a precision of better than 5% under a variety of experimental conditions. The value for M′ is used to calculate true molecular weight values through combination with density values; densities were obtained from outside sources, from ultrasonic densitometry, and from sedimentation coefficients determined independently. The actual molecular weight is also determined through SdFFF analysis of the virus in carriers of different densities. Values for the particle diameter are obtained from SdFFF zone broadening studies. The various SdFFF characterization results are compared with molecular weight, particle size, and density values determined by conventional methods.

Original languageEnglish (US)
Pages (from-to)145-160
Number of pages16
JournalJournal of Virological Methods
Volume11
Issue number2
DOIs
StatePublished - Jun 1985

Keywords

  • PBCV virus sedimentation field flow fractionation Paramecium bursaria

ASJC Scopus subject areas

  • Virology

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