Physical properties of a minimal infectious RNA (viroid) associated with the exocortis disease

J. S. Semancik; T. J. Morris; L. G. Weathers; B. F. Rodorf; D. R. Kearns

doi:10.1016/0042-6822(75)90381-5

Physical properties of a minimal infectious RNA (viroid) associated with the exocortis disease

J. S. Semancik, T. J. Morris, L. G. Weathers, B. F. Rodorf, D. R. Kearns

Biological Sciences

Research output: Contribution to journal › Article › peer-review

94 Scopus citations

Abstract

Purification of the pathogenic RNA (viroid) from exocortis disease has been accomplished. Composition analysis indicates the absence of any DNA or protein and a molar nucleotide composition of CMP, 29.4%; AMP, 21.5%; GMP, 28.8%; and UMP, 19.9%. Even though the AMP UMP and GMP CMP ratios approach unity the per cent hyperchromicity (22%) resembled a tRNA-like molecule and not a dsRNA, while the T_m (52°) in 0.1 SSC was higher than the pure tRNA species tested. Nuclease digestion similarly suggested the presence of a highly ordered structure for the pathogenic RNA. We also introduced low field NMR spectroscopy as a new technique in the study of pathogenic RNA's (viroids) of unknown sequence.

Original language	English (US)
Pages (from-to)	160-167
Number of pages	8
Journal	Virology
Volume	63
Issue number	1
DOIs	https://doi.org/10.1016/0042-6822(75)90381-5
State	Published - Jan 1975

ASJC Scopus subject areas

Virology

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10.1016/0042-6822(75)90381-5

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title = "Physical properties of a minimal infectious RNA (viroid) associated with the exocortis disease",

abstract = "Purification of the pathogenic RNA (viroid) from exocortis disease has been accomplished. Composition analysis indicates the absence of any DNA or protein and a molar nucleotide composition of CMP, 29.4%; AMP, 21.5%; GMP, 28.8%; and UMP, 19.9%. Even though the AMP UMP and GMP CMP ratios approach unity the per cent hyperchromicity (22%) resembled a tRNA-like molecule and not a dsRNA, while the Tm (52°) in 0.1 SSC was higher than the pure tRNA species tested. Nuclease digestion similarly suggested the presence of a highly ordered structure for the pathogenic RNA. We also introduced low field NMR spectroscopy as a new technique in the study of pathogenic RNA's (viroids) of unknown sequence.",

author = "Semancik, {J. S.} and Morris, {T. J.} and Weathers, {L. G.} and Rodorf, {B. F.} and Kearns, {D. R.}",

note = "Funding Information: {\textquoteleft}Supported in part by grants from the National Science Foundation (GB 39605 to JSS, GB 41110 to DRK) and the U.S. Public Health Service GM 19313 to DRK). {\textquoteleft}Submitted in partial fulfillment of the requirements for the Ph.D. degree in chemistry at the University of California, Riverside.",

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doi = "10.1016/0042-6822(75)90381-5",

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AU - Morris, T. J.

AU - Weathers, L. G.

AU - Rodorf, B. F.

AU - Kearns, D. R.

N1 - Funding Information: ‘Supported in part by grants from the National Science Foundation (GB 39605 to JSS, GB 41110 to DRK) and the U.S. Public Health Service GM 19313 to DRK). ‘Submitted in partial fulfillment of the requirements for the Ph.D. degree in chemistry at the University of California, Riverside.

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N2 - Purification of the pathogenic RNA (viroid) from exocortis disease has been accomplished. Composition analysis indicates the absence of any DNA or protein and a molar nucleotide composition of CMP, 29.4%; AMP, 21.5%; GMP, 28.8%; and UMP, 19.9%. Even though the AMP UMP and GMP CMP ratios approach unity the per cent hyperchromicity (22%) resembled a tRNA-like molecule and not a dsRNA, while the Tm (52°) in 0.1 SSC was higher than the pure tRNA species tested. Nuclease digestion similarly suggested the presence of a highly ordered structure for the pathogenic RNA. We also introduced low field NMR spectroscopy as a new technique in the study of pathogenic RNA's (viroids) of unknown sequence.

AB - Purification of the pathogenic RNA (viroid) from exocortis disease has been accomplished. Composition analysis indicates the absence of any DNA or protein and a molar nucleotide composition of CMP, 29.4%; AMP, 21.5%; GMP, 28.8%; and UMP, 19.9%. Even though the AMP UMP and GMP CMP ratios approach unity the per cent hyperchromicity (22%) resembled a tRNA-like molecule and not a dsRNA, while the Tm (52°) in 0.1 SSC was higher than the pure tRNA species tested. Nuclease digestion similarly suggested the presence of a highly ordered structure for the pathogenic RNA. We also introduced low field NMR spectroscopy as a new technique in the study of pathogenic RNA's (viroids) of unknown sequence.

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Physical properties of a minimal infectious RNA (viroid) associated with the exocortis disease

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