Physical properties of a minimal infectious RNA (viroid) associated with the exocortis disease

J. S. Semancik, T. J. Morris, L. G. Weathers, B. F. Rodorf, D. R. Kearns

Research output: Contribution to journalArticle

91 Scopus citations

Abstract

Purification of the pathogenic RNA (viroid) from exocortis disease has been accomplished. Composition analysis indicates the absence of any DNA or protein and a molar nucleotide composition of CMP, 29.4%; AMP, 21.5%; GMP, 28.8%; and UMP, 19.9%. Even though the AMP UMP and GMP CMP ratios approach unity the per cent hyperchromicity (22%) resembled a tRNA-like molecule and not a dsRNA, while the Tm (52°) in 0.1 SSC was higher than the pure tRNA species tested. Nuclease digestion similarly suggested the presence of a highly ordered structure for the pathogenic RNA. We also introduced low field NMR spectroscopy as a new technique in the study of pathogenic RNA's (viroids) of unknown sequence.

Original languageEnglish (US)
Pages (from-to)160-167
Number of pages8
JournalVirology
Volume63
Issue number1
DOIs
StatePublished - Jan 1975

ASJC Scopus subject areas

  • Virology

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    Semancik, J. S., Morris, T. J., Weathers, L. G., Rodorf, B. F., & Kearns, D. R. (1975). Physical properties of a minimal infectious RNA (viroid) associated with the exocortis disease. Virology, 63(1), 160-167. https://doi.org/10.1016/0042-6822(75)90381-5