Physical properties of a minimal infectious RNA (viroid) associated with the exocortis disease

J. S. Semancik; T. J. Morris; L. G. Weathers; B. F. Rodorf; D. R. Kearns

doi:10.1016/0042-6822(75)90381-5

Physical properties of a minimal infectious RNA (viroid) associated with the exocortis disease

J. S. Semancik, T. J. Morris, L. G. Weathers, B. F. Rodorf, D. R. Kearns

Biological Sciences

Research output: Contribution to journal › Article › peer-review

91 Scopus citations

Abstract

Purification of the pathogenic RNA (viroid) from exocortis disease has been accomplished. Composition analysis indicates the absence of any DNA or protein and a molar nucleotide composition of CMP, 29.4%; AMP, 21.5%; GMP, 28.8%; and UMP, 19.9%. Even though the AMP UMP and GMP CMP ratios approach unity the per cent hyperchromicity (22%) resembled a tRNA-like molecule and not a dsRNA, while the T_m (52°) in 0.1 SSC was higher than the pure tRNA species tested. Nuclease digestion similarly suggested the presence of a highly ordered structure for the pathogenic RNA. We also introduced low field NMR spectroscopy as a new technique in the study of pathogenic RNA's (viroids) of unknown sequence.

Original language	English (US)
Pages (from-to)	160-167
Number of pages	8
Journal	Virology
Volume	63
Issue number	1
DOIs	https://doi.org/10.1016/0042-6822(75)90381-5
State	Published - Jan 1975

ASJC Scopus subject areas

Virology

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abstract = "Purification of the pathogenic RNA (viroid) from exocortis disease has been accomplished. Composition analysis indicates the absence of any DNA or protein and a molar nucleotide composition of CMP, 29.4%; AMP, 21.5%; GMP, 28.8%; and UMP, 19.9%. Even though the AMP UMP and GMP CMP ratios approach unity the per cent hyperchromicity (22%) resembled a tRNA-like molecule and not a dsRNA, while the Tm (52°) in 0.1 SSC was higher than the pure tRNA species tested. Nuclease digestion similarly suggested the presence of a highly ordered structure for the pathogenic RNA. We also introduced low field NMR spectroscopy as a new technique in the study of pathogenic RNA's (viroids) of unknown sequence.",

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