Physicochemical and pharmacokinetic characteristics of plasmid DNA/cationic liposome complexes

Ram I. Mahato, Kenji Kawabata, Takehiko Nomura, Yoshinobu Takakura, Mitsuru Hashida

Research output: Contribution to journalArticle

144 Scopus citations

Abstract

The objectives of this study were (i) to characterize the plasmid DNA encoding the chloramphenicol acetyltransferase reporter gene (pCAT) complexed with cationic liposomes (Lipofectin and LipofectACE) in terms of particle size and ζ potential, (ii) to compare pharmacokinetic characteristics, and (iii) to study the hepatic uptake mechanisms. DNA/LipofectACE complexes showed a negative ζ potential of −36 mV at 1:5 w/w ratio, but a positive ζ potential of 19 mV at 1:10 w/w ratio. Lipofectin samples showed a positive ζ potential of 21–28 mV at these ratios. These preparations showed a wide particle size distribution ranging from 600 to 1200 nm. Following intravenous injection of 1:10 w/w ratio [32P]pCAT/liposome complexes at a dose of 0.1 mg DNA/kg into the tail vein of mice, radioactivity was rapidly eliminated from the plasma and almost 50–60% of the dose was taken up by the liver within 5 min after administration. Plasmid DNA/liposome complexes were predominantly taken up by the liver nonparenchymal cells. The hepatic uptake was inhibited by preceding administration of dextran sulfate (DS), but not by polycytidic acid (poly[C]) and polyinosinic acid (poly[I]), suggesting the involvement of a phagocytic process. We suggest that these complexes are preferentially taken up by the liver nonparenchymal cells mainly via Kupffer cell phagocytosis.

Original languageEnglish (US)
Pages (from-to)1267-1271
Number of pages5
JournalJournal of Pharmaceutical Sciences
Volume84
Issue number11
DOIs
StatePublished - Nov 1995

ASJC Scopus subject areas

  • Pharmaceutical Science

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