Plant microRNAs display differential 39 truncation and tailing modifications that are ARGONAUTE1 dependent and conserved across species

Plant small RNAs are 39 methylated by the methyltransferase HUA1 ENHANCER1 (HEN1). In plant hen1 mutants, 39 modifications of small RNAs, including oligo-uridylation (tailing), are associated with accelerated degradation of microRNAs (miRNAs). By sequencing small RNAs of the wild type and hen1 mutants from Arabidopsis thaliana, rice (Oryza sativa), and maize (Zea mays), we found 39 truncation prior to tailing is widespread in these mutants. Moreover, the patterns of miRNA truncation and tailing differ substantially among miRNA families but are conserved across species. The same patterns are also observable in wild-type libraries from a broad range of species, only at lower abundances. ARGONAUTE (AGO1), even with defective slicer activity, can bind these truncated and tailed variants of miRNAs. An ago1 mutation in hen1 suppressed such 39 modifications, indicating that they occur while miRNAs are in association with AGO1, either during or after RNAinduced silencing complex assembly. Our results showed AGO1-bound miRNAs are actively 39 truncated and tailed, possibly reflecting the activity of cofactors acting in conserved patterns in miRNA degradation.