Plasmodium falciparum in vitro drug resistance selections and gene editing

Caroline L. Ng, David A. Fidock

Research output: Chapter in Book/Report/Conference proceedingChapter

1 Scopus citations

Abstract

Malaria continues to be a global health burden, threatening over 40% of the world’s population. Drug resistance in Plasmodium falciparum, the etiological agent of the majority of human malaria cases, is compromising elimination efforts. New approaches to treating drug-resistant malaria benefit from defining resistance liabilities of known antimalarial agents and compounds in development and defining genetic changes that mediate loss of parasite susceptibility. Here, we present protocols for in vitro selection of drug-resistant parasites and for site-directed gene editing of candidate resistance mediators to test for causality.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages123-140
Number of pages18
DOIs
StatePublished - 2019

Publication series

NameMethods in Molecular Biology
Volume2013
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

Keywords

  • CRISPR/Cas9
  • Drug resistance
  • Gene editing
  • Malaria
  • Plasmodium falciparum
  • Selections
  • ZFN

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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    Ng, C. L., & Fidock, D. A. (2019). Plasmodium falciparum in vitro drug resistance selections and gene editing. In Methods in Molecular Biology (pp. 123-140). (Methods in Molecular Biology; Vol. 2013). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-9550-9_9