Platelet membrane glycoproteins IIb and IIIa are substrates of purified pp60c-src protein tyrosine kinase

Duygu Findik, Christoph Reuter, Peter Presek

Research output: Contribution to journalArticle

15 Scopus citations

Abstract

Human platelet glycoproteins IIb and IIIa form the receptor for fibrinogen, von Willebrand factor and fibronectin. Isolated human glycoproteins IIb-IIIa are phosphorylated by purified pp60c-src protein tyrosine kinase. Analysis of the phosphorylated proteins on SDS-PAGE showed that under reducing conditions both phosphoproteins change their relative molecular masses from 135 to 120 kDa and from 97 to 105 kDa, which are characteristic properties of glycoproteins IIb-IIIa. Phosphorylated proteins could be immunoprecipitated with an antiserum against glycoproteins IIb-IIIa but not by control serum. Some kinetic properties of the glycoprotein phosphorylations are also investigated. How the glycoprotein IIb-IIIa complex acquires its receptor activity in stimulated platelets is unknown; however, phosphorylation could be an important mechanism.

Original languageEnglish (US)
Pages (from-to)1-4
Number of pages4
JournalFEBS Letters
Volume262
Issue number1
DOIs
StatePublished - Mar 12 1990

Keywords

  • (Platelet)
  • Glycoprotein IIIa
  • Glycoprotein IIb
  • Integrin
  • Protein tyrosine kinase
  • ppo60

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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