TY - JOUR
T1 - Polyamine and macromolecule levels during spore germination in Rhizopus stolonifer and Botryodiplodia theobromae
AU - Nickerson, Kenneth W.
AU - McCune, Bryan K.
AU - Van Etten, James L.
N1 - Funding Information:
We thank John Welch for the use of a scanning fluorometer and Roger Storck for making his paper available to us prior to publication. This investigation was supported by Public Health Service Grant AI-08057 from the National Institute of Allergy and Infectious Diseases.
PY - 1977/12
Y1 - 1977/12
N2 - Polyamine, RNA, DNA, and protein levels were determined during the germination of Rhizopus stolonifer and Botryodiplodia theobromae spores. Putrescine and spermidine were the only polyamines detected in the spores. In both fungi, the increases in spermidine and RNA roughly paralleled one another. In addition, a large transitory increase in the putrescine pool size was observed in R. stolonifer. Quantitatively, the biosynthetic patterns observed in the two fungi were quite different. In R. stolonifer, macromolecular and polyamine syntheses were continuous from the onset of germination, resulting in several fold increases by the time of germ tube formation. These syntheses were delayed in B. theobromae, however; consequently, the macromolecular and polyamine contents only doubled by the time of germ tube formation.
AB - Polyamine, RNA, DNA, and protein levels were determined during the germination of Rhizopus stolonifer and Botryodiplodia theobromae spores. Putrescine and spermidine were the only polyamines detected in the spores. In both fungi, the increases in spermidine and RNA roughly paralleled one another. In addition, a large transitory increase in the putrescine pool size was observed in R. stolonifer. Quantitatively, the biosynthetic patterns observed in the two fungi were quite different. In R. stolonifer, macromolecular and polyamine syntheses were continuous from the onset of germination, resulting in several fold increases by the time of germ tube formation. These syntheses were delayed in B. theobromae, however; consequently, the macromolecular and polyamine contents only doubled by the time of germ tube formation.
KW - Botryodiplodia theobromae
KW - DNA
KW - RNA
KW - polyamines
KW - protein
KW - spores, Rhizopus stolonifer
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U2 - 10.1016/S0147-5975(77)80007-8
DO - 10.1016/S0147-5975(77)80007-8
M3 - Article
AN - SCOPUS:49449128803
SN - 0147-5975
VL - 1
SP - 317
EP - 322
JO - Experimental Mycology
JF - Experimental Mycology
IS - 4
ER -