Poly(ethylene glycol)-polyethyleneimine NanoGel(TM) particles: Novel drug delivery systems for antisense oligonucleotides

Serguei Vinogradov, Elena Batrakova, Alexander Kabanov

Research output: Contribution to journalArticle

186 Scopus citations

Abstract

A novel type of drug delivery system, termed NanoGel(TM) is proposed. NanoGel(TM) represent particles of a hydrophilic polymer network that were synthesized by cross-linking of polyethyleneimine (PEI) and carbonyldiimidazole-activated poly(ethylene glycol) (PEG) using emulsification/solvent evaporation technique. The resulting NanoGel(TM) was fractionated by gel-permeation chromatography. A major fraction with an average particle size of 120 nm was used in further experiments. Antisense phosphorothioate oligonucleotides (SODN) specific to human mdr1 gene were incorporated in these NanoGel(TM) particles. Loading of NanoGel(TM) particles with SODN resulted in reduction of the particle effective diameter to 80 nm and decreased zeta-potential due to neutralization of the charge of PEI chains by SODN. Accumulation of SODN incorporated in NanoGel(TM) particles in multidrug resistant (MDR) human oral epidermoid carcinoma cells (KBv) was significantly increased compared to the free SODN. Furthermore, efficient transport of SODN-loaded NanoGel(TM) particles across polarized monolayers of human intestinal epithelial cells (Caco-2) was demonstrated. Finally, antisense SODNs incorporated in NanoGel(TM) particles were found to effectively inhibit expression of P-glycoprotein (P-gp) efflux pump in MDR cell lines.

Original languageEnglish (US)
Pages (from-to)291-304
Number of pages14
JournalColloids and Surfaces B: Biointerfaces
Volume16
Issue number1-4
DOIs
StatePublished - Nov 1999

Keywords

  • Antisense oligonucleotide
  • Hydrogel
  • Multidrug resistance
  • Nanoparticles
  • Poly(ethylene glycol)
  • Polyethyleneimine, Drug delivery

ASJC Scopus subject areas

  • Biotechnology
  • Surfaces and Interfaces
  • Physical and Theoretical Chemistry
  • Colloid and Surface Chemistry

Fingerprint Dive into the research topics of 'Poly(ethylene glycol)-polyethyleneimine NanoGel(TM) particles: Novel drug delivery systems for antisense oligonucleotides'. Together they form a unique fingerprint.

  • Cite this