Polypeptide Core of a Human Pancreatic Tumor Mucin Antigen

Michael S. Lan, Michael A. Hollingsworth, Richard S. Metzgar

Research output: Contribution to journalArticle

48 Scopus citations

Abstract

This work describes the molecular properties of the polypeptide core of a human pancreatic mucin antigen isolated from a human pancreatic adenocarcinoma cell line, HPAF. Pancreatic tumor mucin was isolated by a combination of molecular sieve chromatography and CsCl/4 m guanidine-HCl density gradient ultracentrifugation. Trifluoromethane sulfonic acid was used to remove carbohydrate units from purified mucin molecules. A rabbit monospecific polyclonal antibody was generated against pancreatic apomucin and reacted with a Mt < 200,000 species. The antibody binding data indicated that the rabbit antiserum raised against pancreatic apomucin cross-reacted with a breast mucin synthetic peptide. Northern blot and immunodot blot analyses of various cell line extracts revealed that a tandem repeat sequence and a similar mRNA were detected in both pancreatic and breast mucin-producing cell lines. These results suggest that pancreatic apomucin and breast apomucin share some similarity in tandem repeated nucleic acid and protein sequences.

Original languageEnglish (US)
Pages (from-to)2997-3001
Number of pages5
JournalCancer Research
Volume50
Issue number10
StatePublished - May 15 1990

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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