TY - JOUR
T1 - Polypeptide flux through bacterial Hsp70
T2 - DnaK cooperates with trigger factor in chaperoning nascent chains
AU - Teter, S. A.
AU - Houry, W. A.
AU - Ang, D.
AU - Tradler, T.
AU - Rockabrand, D.
AU - Fischer, G.
AU - Blum, P.
AU - Georgopoulos, C.
AU - Hartl, F. U.
PY - 1999
Y1 - 1999
N2 - A role for DnaK, the major E. coli Hsp70, in chaperoning de novo protein folding has remained elusive. Here we show that under nonstress conditions DnaK transiently associates with a wide variety of nascent and newly synthesized polypeptides, with a preference for chains larger than 30 kDa. Deletion of the nonessential gene encoding trigger factor, a ribosome- associated chaperone, results in a doubling of the fraction of nascent polypeptides interacting with DnaK. Combined deletion of the trigger factor and DnaK genes is lethal under normal growth conditions. These findings indicate important, partially overlapping functions of DnaK and trigger factor in de novo protein folding and explain why the loss of either chaperone can be tolerated by E. coli.
AB - A role for DnaK, the major E. coli Hsp70, in chaperoning de novo protein folding has remained elusive. Here we show that under nonstress conditions DnaK transiently associates with a wide variety of nascent and newly synthesized polypeptides, with a preference for chains larger than 30 kDa. Deletion of the nonessential gene encoding trigger factor, a ribosome- associated chaperone, results in a doubling of the fraction of nascent polypeptides interacting with DnaK. Combined deletion of the trigger factor and DnaK genes is lethal under normal growth conditions. These findings indicate important, partially overlapping functions of DnaK and trigger factor in de novo protein folding and explain why the loss of either chaperone can be tolerated by E. coli.
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U2 - 10.1016/S0092-8674(00)80787-4
DO - 10.1016/S0092-8674(00)80787-4
M3 - Article
C2 - 10380927
AN - SCOPUS:0033032592
VL - 97
SP - 755
EP - 765
JO - Cell
JF - Cell
SN - 0092-8674
IS - 6
ER -