@article{828f082c37e44add8a84575868e82c8c,
title = "Porcine pancreatic ductal epithelial cells transformed with KRASG12D and SV40T are tumorigenic",
abstract = "We describe our initial studies in the development of an orthotopic, genetically defined, large animal model of pancreatic cancer. Primary pancreatic epithelial cells were isolated from pancreatic duct of domestic pigs. A transformed cell line was generated from these primary cells with oncogenic KRAS and SV40T. The transformed cell lines outperformed the primary and SV40T immortalized cells in terms of proliferation, population doubling time, soft agar growth, transwell migration and invasion. The transformed cell line grew tumors when injected subcutaneously in nude mice, forming glandular structures and staining for epithelial markers. Future work will include implantation studies of these tumorigenic porcine pancreatic cell lines into the pancreas of allogeneic and autologous pigs. The resultant large animal model of pancreatic cancer could be utilized for preclinical research on diagnostic, interventional, and therapeutic technologies.",
author = "Bailey, {Katie L.} and Cartwright, {Sara B.} and Patel, {Neesha S.} and Neeley Remmers and Lazenby, {Audrey J.} and Hollingsworth, {Michael A.} and Carlson, {Mark A.}",
note = "Funding Information: The authors were funded to perform this work through a grant from the National Cancer Institute, and with seed grants from the University of Nebraska Medical Center and the Buffett Cancer Center of Omaha, Nebraska. Funding Information: This study is the result of work supported in part with resources and the use of facilities at the Omaha VA Medical Center (Nebraska-Western Iowa Health Care System). The work was supported by NIH grant 5R01CA222907 and with seed grants from the University of Nebraska Medical Center and the Buffett Cancer Center of Omaha, Nebraska (to MAC). Portions of this study were presented at the 108th Annual Meeting of the American Association for Cancer Research (AACR) in Washington, D.C., April 1-5, 2017. The authors would like to acknowledge the technical assistance of Gerri Siford and Chris Hansen. The authors also would like to thank Dr. Lawrence B. Schook and Dr. Laurie A. Rund at the University of Illinois for the gift of their plasmid that contained the KRASG12D mutation, along with comments, insights and suggestions for its use in this project. Publisher Copyright: {\textcopyright} 2021, The Author(s).",
year = "2021",
month = dec,
doi = "10.1038/s41598-021-92852-2",
language = "English (US)",
volume = "11",
journal = "Scientific Reports",
issn = "2045-2322",
publisher = "Nature Publishing Group",
number = "1",
}