Pore formation in Schlemm's canal endothelial cells is mechano-sensitive and impaired in glaucoma

Ryan M. Pedrigi, Ritika Gupta, Sietse T. Braakman, W. Daniel Stamer, C. Ross Ethier, Darryl R. Overby

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Increased intraocular pressure (IOP) is the leading risk factor for glaucoma, but the mechanisms of IOP regulation during normalcy and disease are poorly understood. Considerable evidence suggests that Schlemm's canal (SC) endothelial cells may influence IOP by regulating aqueous humor outflow via the formation of trans-endothelial pores. This study employs a biomimetic perfusion system to explore pore formation in SC cells in vitro. Our results show that pore formation increases with increasing pressure drop, occurs only when flow is directed basal-to-apical across the cell layer, and it is reduced in glaucomatous versus normal SC cell lines. These results suggest that pore formation is a biomechanically regulated process and they establish our system as the first in vitro model that captures a specific pathology associated with glaucoma.

Original languageEnglish (US)
Title of host publicationASME 2012 Summer Bioengineering Conference, SBC 2012
Pages1183-1184
Number of pages2
DOIs
StatePublished - 2012
EventASME 2012 Summer Bioengineering Conference, SBC 2012 - Fajardo, Puerto Rico
Duration: Jun 20 2012Jun 23 2012

Publication series

NameASME 2012 Summer Bioengineering Conference, SBC 2012

Conference

ConferenceASME 2012 Summer Bioengineering Conference, SBC 2012
CountryPuerto Rico
CityFajardo
Period6/20/126/23/12

Keywords

  • Biomechanics
  • Intraocular pressure
  • Strain

ASJC Scopus subject areas

  • Bioengineering

Fingerprint Dive into the research topics of 'Pore formation in Schlemm's canal endothelial cells is mechano-sensitive and impaired in glaucoma'. Together they form a unique fingerprint.

Cite this