Potential of Pseudomonas sp. JH 51-2 to stabilize lead mining site soil

Jaehong Shim, Patrick J. Shea, Ik Boo Jung, Byung Taek Oh, Min Cho

Research output: Contribution to journalArticlepeer-review

1 Scopus citations


The potential of a lead (Pb)-tolerant Pseudomonas sp. JH 51-2 strain to promote Pb stabilization was evaluated in mining site soil. The strain was isolated from soil containing multiple heavy metals. Transmission electron microscopy (TEM) showed that cell walls were surrounded by extracellular substances and scanning electron microscope-energy dispersive spectroscopy (SEM-EDS) confirmed the presence of Pb on the surface of cell membrane. Fourier transform infrared spectroscopy (FTIR) revealed that amino acids, phospholipids and extracellular polysaccharides were involved in Pb complexation and biosorption. Sequential extraction and toxicity characteristic leaching procedure (TCLP) showed decreased Pb availability in mining site soil in the presence of Pseudomonas sp. JH 51-2. Results indicate that Pseudomonas sp. JH 51-2 is an efficient biological stabilizer of Pb in contaminated soil.

Original languageEnglish (US)
Pages (from-to)e695-e701
JournalJournal of Environmental Biology
Issue number3
StatePublished - May 1 2015


  • Biostabilization
  • Lead
  • Pseudomonas sp.
  • Sequential extraction
  • Toxicity characteristic leaching procedure

ASJC Scopus subject areas

  • Environmental Engineering
  • Toxicology
  • Health, Toxicology and Mutagenesis

Fingerprint Dive into the research topics of 'Potential of Pseudomonas sp. JH 51-2 to stabilize lead mining site soil'. Together they form a unique fingerprint.

Cite this