The potential of a lead (Pb)-tolerant Pseudomonas sp. JH 51-2 strain to promote Pb stabilization was evaluated in mining site soil. The strain was isolated from soil containing multiple heavy metals. Transmission electron microscopy (TEM) showed that cell walls were surrounded by extracellular substances and scanning electron microscope-energy dispersive spectroscopy (SEM-EDS) confirmed the presence of Pb on the surface of cell membrane. Fourier transform infrared spectroscopy (FTIR) revealed that amino acids, phospholipids and extracellular polysaccharides were involved in Pb complexation and biosorption. Sequential extraction and toxicity characteristic leaching procedure (TCLP) showed decreased Pb availability in mining site soil in the presence of Pseudomonas sp. JH 51-2. Results indicate that Pseudomonas sp. JH 51-2 is an efficient biological stabilizer of Pb in contaminated soil.
|Original language||English (US)|
|Journal||Journal of Environmental Biology|
|State||Published - May 1 2015|
- Pseudomonas sp.
- Sequential extraction
- Toxicity characteristic leaching procedure
ASJC Scopus subject areas
- Environmental Engineering
- Health, Toxicology and Mutagenesis