Ppoduction of new WAP-8294A Cyclodepsipeptides by the biological control agent lysobacter enzymogenes OH11

Jing Zhu, Yuan Chen, Liangcheng Du

Research output: Contribution to journalArticlepeer-review

1 Scopus citations


Naturally-occurring environmental microorganisms may provide ‘green’ and effective biocontrol tools for disease management in agricultural crops. Due to the constant threat of resistant pathogens there is a pressing and continual need to search for new biocontrol tools. This study investigated the production of new analogs of WAP-8294A compounds by the biocontrol agent Lysobacter enzymogenes OH11 through biosynthetic engineering. WAP-8294As are a family of natural cyclic lipodepsipeptides with potent activity against Grampositive bacteria. A series of genetic manipulations was therefore conducted on the accessory genes in the WAP biosynthetic gene cluster. The resulting strains containing a single-point mutation in ORF4, which was predicted to encode a 2-ketoglutarate dependent dioxygenase, produced deoxy-WAP- 8294As. This result provides evidence for the function of ORF4 in catalyzing β- hydroxylation of the D-asparagine residue in WAP-8294As. In addition, six new analogs of WAP-8294As were identified by UHPLC-HR-MS/MS. This is the first attempt to produce new WAP-8294As in Lysobacter and shows that the spectrum of the biocontrol compounds may be expanded through the manipulation of biosynthetic genes.

Original languageEnglish (US)
Pages (from-to)120-132
Number of pages13
JournalFrontiers of Agricultural Science and Engineering
Issue number1
StatePublished - Mar 2022


  • Biocontrol
  • Biosynthesis
  • Lysobacter
  • Natural products
  • Wap-8294a

ASJC Scopus subject areas

  • Biotechnology
  • General Veterinary
  • General Agricultural and Biological Sciences


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