TY - JOUR
T1 - Preliminary X-ray diffraction studies of the transcriptional inhibitory antibody Fab41.4
AU - Mazlo, J.
AU - Stanfield, R. L.
AU - Wilson, I. A.
AU - Hinrichs, S. H.
AU - Stezowski, J. J.
PY - 2001
Y1 - 2001
N2 - The binding of transcription factor ATF-1 to DNA contributes to gene expression and regulation of cell growth. Antibody Mab41.4, raised against ATF-1, and its derivatives Fab41.4 and scFv41.4 inhibit specific DNA binding in vitro and induce apoptotic death of tumor cells in vivo. Structural studies of Fab41.4 were performed to gain insight into the mechanism of action of this potentially therapeutic antibody. The optimal conditions for crystallization of Fab41.4 were determined. Crystals were needle-like in appearance, displayed C2 space-group symmetry and diffracted to a resolution of 1.6 Å. The unit-cell parameters were determined to be a = 186.64, b = 40.22, c = 55.58 Å, α = γ = 90, β = 96.93°. The data set was 97.7% complete. Molecular replacement was performed, resulting in an R value of 44.6%.
AB - The binding of transcription factor ATF-1 to DNA contributes to gene expression and regulation of cell growth. Antibody Mab41.4, raised against ATF-1, and its derivatives Fab41.4 and scFv41.4 inhibit specific DNA binding in vitro and induce apoptotic death of tumor cells in vivo. Structural studies of Fab41.4 were performed to gain insight into the mechanism of action of this potentially therapeutic antibody. The optimal conditions for crystallization of Fab41.4 were determined. Crystals were needle-like in appearance, displayed C2 space-group symmetry and diffracted to a resolution of 1.6 Å. The unit-cell parameters were determined to be a = 186.64, b = 40.22, c = 55.58 Å, α = γ = 90, β = 96.93°. The data set was 97.7% complete. Molecular replacement was performed, resulting in an R value of 44.6%.
UR - http://www.scopus.com/inward/record.url?scp=0035098319&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0035098319&partnerID=8YFLogxK
U2 - 10.1107/S0907444901001135
DO - 10.1107/S0907444901001135
M3 - Article
C2 - 11223533
AN - SCOPUS:0035098319
SN - 0907-4449
VL - 57
SP - 462
EP - 464
JO - Acta Crystallographica Section D: Biological Crystallography
JF - Acta Crystallographica Section D: Biological Crystallography
IS - 3
ER -