Preparation and binding of radioactively labeled porcine transforming growth factor type β

Eric Ruff, Angie Rizzino

Research output: Contribution to journalArticlepeer-review

57 Scopus citations

Abstract

This report describes the labeling of porcine transforming growth factor type β (TGF-β) with 125-iodine. Its binding to NRK cells and three other cell lines has been examined. The data indicate that NRK cells exhibit approximately 10,000 receptors for porcine TGF-β per cell, with an apparent dissociation constant of 45 pM. The binding of porcine 125I-TGF-β can be blocked by porcine, murine and human TGF-β but not by several well characterized growth factors. In all respects examined, the binding observed with porcine 125I-TGF-β appears to be the same as that observed with human TGF-β. The findings reported here argue that porcine 125I-TGF-β can be used to quantitate TGF-β receptors on a wide range of mammalian cells.

Original languageEnglish (US)
Pages (from-to)714-719
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume138
Issue number2
DOIs
StatePublished - Jul 31 1986

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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