Preparation of collagen substrates for cell attachment: Effect of collagen concentration and phosphate buffer

Hynda K. Kleinman, Ermona B. McGoodwin, Stephen I. Rennard, George R. Martin

Research output: Contribution to journalArticle

90 Scopus citations

Abstract

We have studied the attachment of cultured Chinese hamster ovary cells to collagen substrates prepared in several ways. The attachment of these cells to collagen required under most conditions either serum or fibronectin purified from serum. Reconstituted collagen substrates required greater amounts of fibronectin than dishes coated by drying a collagen solution, but in each case the amount of fibronectin required was proportional to the amount of collagen on the dish. High levels of phosphate, 0.01 m and above, used as a buffer in heat-reconstituted collagen substrates allowed cell attachment without fibronectin. However, since the cells did not spread under these conditions and were not released from the substrate when incubated with trypsin, binding of cells with such levels of phosphate probably represents nonphysiological adhesion.

Original languageEnglish (US)
Pages (from-to)308-312
Number of pages5
JournalAnalytical Biochemistry
Volume94
Issue number2
DOIs
Publication statusPublished - Apr 15 1979

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ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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