TY - JOUR
T1 - Preparation of collagen substrates for cell attachment
T2 - Effect of collagen concentration and phosphate buffer
AU - Kleinman, Hynda K.
AU - McGoodwin, Ermona B.
AU - Rennard, Stephen I.
AU - Martin, George R.
PY - 1979/4/15
Y1 - 1979/4/15
N2 - We have studied the attachment of cultured Chinese hamster ovary cells to collagen substrates prepared in several ways. The attachment of these cells to collagen required under most conditions either serum or fibronectin purified from serum. Reconstituted collagen substrates required greater amounts of fibronectin than dishes coated by drying a collagen solution, but in each case the amount of fibronectin required was proportional to the amount of collagen on the dish. High levels of phosphate, 0.01 m and above, used as a buffer in heat-reconstituted collagen substrates allowed cell attachment without fibronectin. However, since the cells did not spread under these conditions and were not released from the substrate when incubated with trypsin, binding of cells with such levels of phosphate probably represents nonphysiological adhesion.
AB - We have studied the attachment of cultured Chinese hamster ovary cells to collagen substrates prepared in several ways. The attachment of these cells to collagen required under most conditions either serum or fibronectin purified from serum. Reconstituted collagen substrates required greater amounts of fibronectin than dishes coated by drying a collagen solution, but in each case the amount of fibronectin required was proportional to the amount of collagen on the dish. High levels of phosphate, 0.01 m and above, used as a buffer in heat-reconstituted collagen substrates allowed cell attachment without fibronectin. However, since the cells did not spread under these conditions and were not released from the substrate when incubated with trypsin, binding of cells with such levels of phosphate probably represents nonphysiological adhesion.
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U2 - 10.1016/0003-2697(79)90365-8
DO - 10.1016/0003-2697(79)90365-8
M3 - Article
C2 - 464299
AN - SCOPUS:0018356117
VL - 94
SP - 308
EP - 312
JO - Analytical Biochemistry
JF - Analytical Biochemistry
SN - 0003-2697
IS - 2
ER -