Preparation of DNA and nucleoprotein samples for AFM imaging

Research output: Contribution to journalReview article

59 Scopus citations

Abstract

Sample preparation techniques allowing reliable and reproducible imaging of DNA with various structures, topologies and complexes with proteins are reviewed. The major emphasis is given to methods utilizing chemical functionalization of mica, enabling preparation of the surfaces with required characteristics. The methods are illustrated by examples of imaging of different DNA structures. Special attention is given to the possibility of AFM to image the dynamics of DNA at the nanoscale. The capabilities of time-lapse AFM in aqueous solutions are illustrated by imaging of dynamic processes as transitions of local alternative structures (transition of DNA between H and B forms). The application of AFM to studies of protein-DNA complexes is illustrated by a few examples of imaging site-specific complexes, as well as such systems as chromatin. The time-lapse AFM studies of protein-DNA complexes including very recent advances with the use of high-speed AFM are reviewed.

Original languageEnglish (US)
Pages (from-to)196-206
Number of pages11
JournalMicron
Volume42
Issue number2
DOIs
StatePublished - Feb 2011

Keywords

  • AFM
  • Alternative DNA structures
  • DNA
  • DNA supercoiling
  • Protein-DNA complexes
  • Silanes
  • Single molecule imaging
  • Surface functionalization

ASJC Scopus subject areas

  • Structural Biology
  • Materials Science(all)
  • Physics and Astronomy(all)
  • Cell Biology

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