Preparation of high-capacity supports containing protein G immobilized to porous silica

Abby J. Jackson; Elizabeth M. Karle; David S. Hage

doi:10.1016/j.ab.2010.07.004

Preparation of high-capacity supports containing protein G immobilized to porous silica

Abby J. Jackson, Elizabeth M. Karle, David S. Hage

Research output: Contribution to journal › Article › peer-review

15 Scopus citations

Abstract

This study examined the preparation of high-capacity silica supports containing immobilized protein G. A maximum content of 39mg protein G/g silica was obtained when using 100Å pore size silica, followed by 33mg/g for 50Å silica and 9.3-24mg/g for 300-4000Å silica. The surface coverage of protein G increased with pore size, with a maximum level of 0.037μmol/m² being obtained for 4000Å silica. These supports gave comparable apparent activities (i.e., 30-47% binding to rabbit immunoglobulin G [IgG]), with the highest binding capacities (71-77mg IgG/g silica) being obtained for 50-100Å silica.

Original language	English (US)
Pages (from-to)	235-237
Number of pages	3
Journal	Analytical Biochemistry
Volume	406
Issue number	2
DOIs	https://doi.org/10.1016/j.ab.2010.07.004
State	Published - Nov 2010
Externally published	Yes

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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title = "Preparation of high-capacity supports containing protein G immobilized to porous silica",

abstract = "This study examined the preparation of high-capacity silica supports containing immobilized protein G. A maximum content of 39mg protein G/g silica was obtained when using 100{\AA} pore size silica, followed by 33mg/g for 50{\AA} silica and 9.3-24mg/g for 300-4000{\AA} silica. The surface coverage of protein G increased with pore size, with a maximum level of 0.037μmol/m2 being obtained for 4000{\AA} silica. These supports gave comparable apparent activities (i.e., 30-47% binding to rabbit immunoglobulin G [IgG]), with the highest binding capacities (71-77mg IgG/g silica) being obtained for 50-100{\AA} silica.",

author = "Jackson, {Abby J.} and Karle, {Elizabeth M.} and Hage, {David S.}",

note = "Funding Information: This research was supported by the National Institutes of Health (NIH) under grant R01 GM044931 and was conducted in facilities that were renovated under NIH grant RR015468-01 . ",

year = "2010",

month = nov,

doi = "10.1016/j.ab.2010.07.004",

language = "English (US)",

volume = "406",

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journal = "Analytical Biochemistry",

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T1 - Preparation of high-capacity supports containing protein G immobilized to porous silica

AU - Jackson, Abby J.

AU - Karle, Elizabeth M.

AU - Hage, David S.

N1 - Funding Information: This research was supported by the National Institutes of Health (NIH) under grant R01 GM044931 and was conducted in facilities that were renovated under NIH grant RR015468-01 .

PY - 2010/11

Y1 - 2010/11

N2 - This study examined the preparation of high-capacity silica supports containing immobilized protein G. A maximum content of 39mg protein G/g silica was obtained when using 100Å pore size silica, followed by 33mg/g for 50Å silica and 9.3-24mg/g for 300-4000Å silica. The surface coverage of protein G increased with pore size, with a maximum level of 0.037μmol/m2 being obtained for 4000Å silica. These supports gave comparable apparent activities (i.e., 30-47% binding to rabbit immunoglobulin G [IgG]), with the highest binding capacities (71-77mg IgG/g silica) being obtained for 50-100Å silica.

AB - This study examined the preparation of high-capacity silica supports containing immobilized protein G. A maximum content of 39mg protein G/g silica was obtained when using 100Å pore size silica, followed by 33mg/g for 50Å silica and 9.3-24mg/g for 300-4000Å silica. The surface coverage of protein G increased with pore size, with a maximum level of 0.037μmol/m2 being obtained for 4000Å silica. These supports gave comparable apparent activities (i.e., 30-47% binding to rabbit immunoglobulin G [IgG]), with the highest binding capacities (71-77mg IgG/g silica) being obtained for 50-100Å silica.

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JO - Analytical Biochemistry

JF - Analytical Biochemistry

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Preparation of high-capacity supports containing protein G immobilized to porous silica

Abstract

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