Preparation of high-capacity supports containing protein G immobilized to porous silica

Abby J. Jackson, Elizabeth M. Karle, David S. Hage

Research output: Contribution to journalArticle

11 Scopus citations

Abstract

This study examined the preparation of high-capacity silica supports containing immobilized protein G. A maximum content of 39mg protein G/g silica was obtained when using 100Å pore size silica, followed by 33mg/g for 50Å silica and 9.3-24mg/g for 300-4000Å silica. The surface coverage of protein G increased with pore size, with a maximum level of 0.037μmol/m2 being obtained for 4000Å silica. These supports gave comparable apparent activities (i.e., 30-47% binding to rabbit immunoglobulin G [IgG]), with the highest binding capacities (71-77mg IgG/g silica) being obtained for 50-100Å silica.

Original languageEnglish (US)
Pages (from-to)235-237
Number of pages3
JournalAnalytical Biochemistry
Volume406
Issue number2
DOIs
StatePublished - Nov 2010

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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