TY - JOUR
T1 - Prevalence of germinal center B-cell-like and non-germinal center B-cell-like types of diffuse large B-cell lymphoma in Shanghai, China
AU - Chen, Yan
AU - Chen, Hui
AU - Fu, Kai
AU - Zhu, Xiong Zeng
AU - Richard, Irons
PY - 2010/5
Y1 - 2010/5
N2 - Objective: To study the prevalence of germinal center B-cell-like (GCB) and non-GCB-like types of diffuse large B-cell lymphoma (DLBCL) in Chinese patients. Methods: Immunohistochemical study for CD10, bcl-6, MUM1, GCET1 and F0XP1 was performed on 124 cases of DLBCL from Shanghai, China. The Hans algorithm and Choi algorithm were applied to classify DLBCL into GCB and non-GCB-like types. Fluorescence in-situ hybridization (FISH) for t (14; 18) and bcl-6 gene rearrangement was also carried out on 118 cases. Results: Of the 124 DLBCL cases studied, 27 cases (22%) showed a GCB-like type and 97 cases (78%) showed a non-GCB-like type, when using Hans algorithm. On the other hand, 34 cases (27%) belonged to GCB-like type and 90 cases (73%) belonged to non-GCB-like type when applying Choi algorithm. The prevalence of GCB-like type was significantly lower than that of non-GCB-like type (P = 0.0001). Only four cases (3%) were positive for t (14; 18), and three of them were classified as GCB-like type. bcl-6 rearrangement was found in 46 cases (39%) and more frequently encountered in the GCB-like type. There is no relationship between bcl-6 gene rearrangement and bcl-6 protein expression. Conclusions: The GCB-like type of DLBCL is significantly less common than non-GCB-like type in Chinese population. This phenomenon is possibly related to the low frequency of t(14;18).
AB - Objective: To study the prevalence of germinal center B-cell-like (GCB) and non-GCB-like types of diffuse large B-cell lymphoma (DLBCL) in Chinese patients. Methods: Immunohistochemical study for CD10, bcl-6, MUM1, GCET1 and F0XP1 was performed on 124 cases of DLBCL from Shanghai, China. The Hans algorithm and Choi algorithm were applied to classify DLBCL into GCB and non-GCB-like types. Fluorescence in-situ hybridization (FISH) for t (14; 18) and bcl-6 gene rearrangement was also carried out on 118 cases. Results: Of the 124 DLBCL cases studied, 27 cases (22%) showed a GCB-like type and 97 cases (78%) showed a non-GCB-like type, when using Hans algorithm. On the other hand, 34 cases (27%) belonged to GCB-like type and 90 cases (73%) belonged to non-GCB-like type when applying Choi algorithm. The prevalence of GCB-like type was significantly lower than that of non-GCB-like type (P = 0.0001). Only four cases (3%) were positive for t (14; 18), and three of them were classified as GCB-like type. bcl-6 rearrangement was found in 46 cases (39%) and more frequently encountered in the GCB-like type. There is no relationship between bcl-6 gene rearrangement and bcl-6 protein expression. Conclusions: The GCB-like type of DLBCL is significantly less common than non-GCB-like type in Chinese population. This phenomenon is possibly related to the low frequency of t(14;18).
KW - Immunophenotyping
KW - Lymphoma, B-cell
KW - Proto-oncogene proteins c-bcl-6
KW - Translocation, genetic
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U2 - 10.3760/cma.j.issn.0529-5807.2010.05.006
DO - 10.3760/cma.j.issn.0529-5807.2010.05.006
M3 - Article
C2 - 20654154
AN - SCOPUS:77953090212
SN - 0529-5807
VL - 39
SP - 313
EP - 318
JO - Chinese Journal of Pathology
JF - Chinese Journal of Pathology
IS - 5
ER -