Prevalence of non-O157:H7 Shiga toxin-producing Escherichia coli in diarrheal stool samples from Nebraska

Paul D. Fey; R. S. Wickert; M. E. Rupp; T. J. Safranek; S. H. Hinrichs

doi:10.3201/eid0605.000513

Prevalence of non-O157:H7 Shiga toxin-producing Escherichia coli in diarrheal stool samples from Nebraska

Paul D. Fey, R. S. Wickert, M. E. Rupp, T. J. Safranek, S. H. Hinrichs

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113 Scopus citations

Abstract

We determined the prevalence of Shiga toxin-producing Escherichia coli (STEC) in diarrheal stool samples from Nebraska by three methods: cefixime-tellurite sorbitol MacConkey (CT-SMAC) culture, enterohemorrhagic E. coli (EHEC) enzyme immunoassay, and stx_1,2 polymerase chain reaction (PCR). Fourteen (4.2%) of 335 specimens were positive by at least one method (CT-SMAC culture [6 of 14], EHEC enzyme immunoassay [13 of 14], stx_1,2PCR [14 of 14]). Six contained serogroup 0157, while non-0157 were as prevalent as 0157 serogroups.

Original language	English (US)
Pages (from-to)	530-533
Number of pages	4
Journal	Emerging infectious diseases
Volume	6
Issue number	5
DOIs	https://doi.org/10.3201/eid0605.000513
State	Published - 2000

ASJC Scopus subject areas

Epidemiology
Microbiology (medical)
Infectious Diseases

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abstract = "We determined the prevalence of Shiga toxin-producing Escherichia coli (STEC) in diarrheal stool samples from Nebraska by three methods: cefixime-tellurite sorbitol MacConkey (CT-SMAC) culture, enterohemorrhagic E. coli (EHEC) enzyme immunoassay, and stx1,2 polymerase chain reaction (PCR). Fourteen (4.2%) of 335 specimens were positive by at least one method (CT-SMAC culture [6 of 14], EHEC enzyme immunoassay [13 of 14], stx1,2PCR [14 of 14]). Six contained serogroup 0157, while non-0157 were as prevalent as 0157 serogroups.",

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AU - Hinrichs, S. H.

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Prevalence of non-O157:H7 Shiga toxin-producing Escherichia coli in diarrheal stool samples from Nebraska

Abstract

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