TY - CHAP
T1 - Principles and applications of immunoaffinity chromatography
AU - Jackson, Abby J.
AU - Sobansky, Matthew R.
AU - Hage, David S.
N1 - Copyright:
Copyright 2013 Elsevier B.V., All rights reserved.
PY - 2012
Y1 - 2012
N2 - Immunoaffinity Chromatography (IAC) is a type of liquid chromatography that uses the highly specific binding of antibodies for the retention of a target. This approach can be used in a myriad of techniques for the selective purification, concentration and/or analysis of target compounds. This chapter discusses the basic components of an IAC method, including the support, stationary phase, immobilization technique, and proper sample application and elution conditions. Several applications of IAC are also considered. These applications include immunoextraction, immunodepletion, chromatographic immunoassays, and post-column immunodetection. A wide range of targets that have been isolated or measured by these techniques are listed. Related methods based on antibody mimics are also discussed.
AB - Immunoaffinity Chromatography (IAC) is a type of liquid chromatography that uses the highly specific binding of antibodies for the retention of a target. This approach can be used in a myriad of techniques for the selective purification, concentration and/or analysis of target compounds. This chapter discusses the basic components of an IAC method, including the support, stationary phase, immobilization technique, and proper sample application and elution conditions. Several applications of IAC are also considered. These applications include immunoextraction, immunodepletion, chromatographic immunoassays, and post-column immunodetection. A wide range of targets that have been isolated or measured by these techniques are listed. Related methods based on antibody mimics are also discussed.
KW - Antibodies
KW - Immunoaffinity chromatography
UR - http://www.scopus.com/inward/record.url?scp=84887367280&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84887367280&partnerID=8YFLogxK
U2 - 10.2174/978160805264611201010156
DO - 10.2174/978160805264611201010156
M3 - Chapter
AN - SCOPUS:84887367280
SN - 9781608055869
SP - 156
EP - 174
BT - Antibodies Applications and New Development
PB - Bentham Science Publishers Ltd.
ER -