Principles and applications of immunoaffinity chromatography

Abby J. Jackson; Matthew R. Sobansky; David S. Hage

doi:10.2174/978160805264611201010156

Principles and applications of immunoaffinity chromatography

Abby J. Jackson, Matthew R. Sobansky, David S. Hage

Chemistry

Research output: Chapter in Book/Report/Conference proceeding › Chapter

1 Scopus citations

Abstract

Immunoaffinity Chromatography (IAC) is a type of liquid chromatography that uses the highly specific binding of antibodies for the retention of a target. This approach can be used in a myriad of techniques for the selective purification, concentration and/or analysis of target compounds. This chapter discusses the basic components of an IAC method, including the support, stationary phase, immobilization technique, and proper sample application and elution conditions. Several applications of IAC are also considered. These applications include immunoextraction, immunodepletion, chromatographic immunoassays, and post-column immunodetection. A wide range of targets that have been isolated or measured by these techniques are listed. Related methods based on antibody mimics are also discussed.

Original language	English (US)
Title of host publication	Antibodies Applications and New Development
Publisher	Bentham Science Publishers Ltd.
Pages	156-174
Number of pages	19
ISBN (Print)	9781608055869
DOIs	https://doi.org/10.2174/978160805264611201010156
State	Published - Dec 1 2012

Keywords

Antibodies
Immunoaffinity chromatography

ASJC Scopus subject areas

Medicine(all)

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Jackson, A. J., Sobansky, M. R., & Hage, D. S. (2012). Principles and applications of immunoaffinity chromatography. In Antibodies Applications and New Development (pp. 156-174). Bentham Science Publishers Ltd.. https://doi.org/10.2174/978160805264611201010156

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