Principles of rapid polymerase chain reaction: Mathematical modeling and experimental verification

H. J. Viljoen, R. M. Nelson, S. Whitney

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

The principles of rapid polymerase chain reaction (PCR) by mathematical modeling and experimental verification was discussed. The amplification of DNA sample by PCR consisted of separation of double stranded DNA at elevated temperatures to form single stranded DNA. The PCRJet™thermocycles has set speed records for every DNA amplicon tested. Viral and bacterial DNA fragments 100 to 500 b.p. long were amplified through 30 PCR cycles in 2 to 4 minutes, whereas single copy human gene fragments in this size range required 3,5 to 6,0 minutes for 35 PCR cycles.

Original languageEnglish (US)
Title of host publicationAIChE Annual Meeting, Conference Proceedings
Pages7573-7589
Number of pages17
StatePublished - 2004
Event2004 AIChE Annual Meeting - Austin, TX
Duration: Nov 7 2004Nov 12 2004

Other

Other2004 AIChE Annual Meeting
CityAustin, TX
Period11/7/0411/12/04

ASJC Scopus subject areas

  • Engineering(all)

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  • Cite this

    Viljoen, H. J., Nelson, R. M., & Whitney, S. (2004). Principles of rapid polymerase chain reaction: Mathematical modeling and experimental verification. In AIChE Annual Meeting, Conference Proceedings (pp. 7573-7589)