Abstract
The principles of rapid polymerase chain reaction (PCR) by mathematical modeling and experimental verification was discussed. The amplification of DNA sample by PCR consisted of separation of double stranded DNA at elevated temperatures to form single stranded DNA. The PCRJet™thermocycles has set speed records for every DNA amplicon tested. Viral and bacterial DNA fragments 100 to 500 b.p. long were amplified through 30 PCR cycles in 2 to 4 minutes, whereas single copy human gene fragments in this size range required 3,5 to 6,0 minutes for 35 PCR cycles.
| Original language | English (US) |
|---|---|
| Title of host publication | AIChE Annual Meeting, Conference Proceedings |
| Pages | 7573-7589 |
| Number of pages | 17 |
| State | Published - 2004 |
| Event | 2004 AIChE Annual Meeting - Austin, TX Duration: Nov 7 2004 → Nov 12 2004 |
Other
| Other | 2004 AIChE Annual Meeting |
|---|---|
| City | Austin, TX |
| Period | 11/7/04 → 11/12/04 |
ASJC Scopus subject areas
- General Engineering