Pristane induced gene activation

Lenora R. Garrett, Clinton J. Jones, Marvin A. Cuchens

Research output: Contribution to journalArticlepeer-review

8 Scopus citations

Abstract

Studies were performed to examine the effects of 2,6,10,14-tetramethyl pentadecane (pristane) versus 12-O-tetradecanoylphorbol 13-acetate (TPA) on the activation of the CAT gene under the regulatory control of viral promoter/enhancer elements transfected into NIH-3T3, CV-1 and COS-7 cells. The results of these studies demonstrated that (1) pristane or TPA induced trans-activation of SV2cat, HIVcat, RSVcat and MMTVcat in cells transfected with each respective plasmid construct, (2) only pristane induced activation of pA10cat and pOSP/11 and (3) neither TPA nor pristane trans-activated pSV0cat. Furthermore, treatment with either pristane or TPA elicited changes in the morphology of each of the cell lines. Collectively these results indicate that pristane is a potent inducer of gene expression and exhibits similar characteristics as the tumor promoter, TPA.

Original languageEnglish (US)
Pages (from-to)119-130
Number of pages12
JournalChemico-Biological Interactions
Volume81
Issue number1-2
DOIs
StatePublished - Jan 1992

Keywords

  • 12-O-tetradecanoylphorbol 13-acetate
  • Chloramphenicol acetyltransferase
  • Pristane
  • Transfection
  • trans-Activation

ASJC Scopus subject areas

  • Toxicology

Fingerprint Dive into the research topics of 'Pristane induced gene activation'. Together they form a unique fingerprint.

Cite this