Pristane induced gene activation

Lenora R. Garrett; Clinton J. Jones; Marvin A. Cuchens

doi:10.1016/0009-2797(92)90030-O

Pristane induced gene activation

Lenora R. Garrett, Clinton J. Jones, Marvin A. Cuchens

Research output: Contribution to journal › Article › peer-review

2 Scopus citations

Abstract

Studies were performed to examine the effects of 2,6,10,14-tetramethyl pentadecane (pristane) versus 12-O-tetradecanoylphorbol 13-acetate (TPA) on the activation of the CAT gene under the regulatory control of viral promoter/enhancer elements transfected into NIH-3T3, CV-1 and COS-7 cells. The results of these studies demonstrated that (1) pristane or TPA induced trans-activation of SV2cat, HIVcat, RSVcat and MMTVcat in cells transfected with each respective plasmid construct, (2) only pristane induced activation of pA10cat and pOSP/11 and (3) neither TPA nor pristane trans-activated pSV0cat. Furthermore, treatment with either pristane or TPA elicited changes in the morphology of each of the cell lines. Collectively these results indicate that pristane is a potent inducer of gene expression and exhibits similar characteristics as the tumor promoter, TPA.

Original language	English (US)
Pages (from-to)	119-130
Number of pages	12
Journal	Chemico-Biological Interactions
Volume	81
Issue number	1-2
DOIs	https://doi.org/10.1016/0009-2797(92)90030-O
State	Published - Jan 1992
Externally published	Yes

Keywords

12-O-tetradecanoylphorbol 13-acetate
Chloramphenicol acetyltransferase
Pristane
Transfection
trans-Activation

ASJC Scopus subject areas

Toxicology

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10.1016/0009-2797(92)90030-O

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@article{1ded0d7cfab24e8ab545e7fd6a62213f,

title = "Pristane induced gene activation",

abstract = "Studies were performed to examine the effects of 2,6,10,14-tetramethyl pentadecane (pristane) versus 12-O-tetradecanoylphorbol 13-acetate (TPA) on the activation of the CAT gene under the regulatory control of viral promoter/enhancer elements transfected into NIH-3T3, CV-1 and COS-7 cells. The results of these studies demonstrated that (1) pristane or TPA induced trans-activation of SV2cat, HIVcat, RSVcat and MMTVcat in cells transfected with each respective plasmid construct, (2) only pristane induced activation of pA10cat and pOSP/11 and (3) neither TPA nor pristane trans-activated pSV0cat. Furthermore, treatment with either pristane or TPA elicited changes in the morphology of each of the cell lines. Collectively these results indicate that pristane is a potent inducer of gene expression and exhibits similar characteristics as the tumor promoter, TPA.",

keywords = "12-O-tetradecanoylphorbol 13-acetate, Chloramphenicol acetyltransferase, Pristane, Transfection, trans-Activation",

author = "Garrett, {Lenora R.} and Jones, {Clinton J.} and Cuchens, {Marvin A.}",

note = "Funding Information: This investigation was supported by PHS grant numbers CA33111 (M.A.C.) and CA47872 (C.J.J.) from the National Cancer Institute.",

year = "1992",

month = jan,

doi = "10.1016/0009-2797(92)90030-O",

language = "English (US)",

volume = "81",

pages = "119--130",

journal = "Chemico-Biological Interactions",

issn = "0009-2797",

publisher = "Elsevier Ireland Ltd",

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}

TY - JOUR

T1 - Pristane induced gene activation

AU - Garrett, Lenora R.

AU - Jones, Clinton J.

AU - Cuchens, Marvin A.

N1 - Funding Information: This investigation was supported by PHS grant numbers CA33111 (M.A.C.) and CA47872 (C.J.J.) from the National Cancer Institute.

PY - 1992/1

Y1 - 1992/1

N2 - Studies were performed to examine the effects of 2,6,10,14-tetramethyl pentadecane (pristane) versus 12-O-tetradecanoylphorbol 13-acetate (TPA) on the activation of the CAT gene under the regulatory control of viral promoter/enhancer elements transfected into NIH-3T3, CV-1 and COS-7 cells. The results of these studies demonstrated that (1) pristane or TPA induced trans-activation of SV2cat, HIVcat, RSVcat and MMTVcat in cells transfected with each respective plasmid construct, (2) only pristane induced activation of pA10cat and pOSP/11 and (3) neither TPA nor pristane trans-activated pSV0cat. Furthermore, treatment with either pristane or TPA elicited changes in the morphology of each of the cell lines. Collectively these results indicate that pristane is a potent inducer of gene expression and exhibits similar characteristics as the tumor promoter, TPA.

AB - Studies were performed to examine the effects of 2,6,10,14-tetramethyl pentadecane (pristane) versus 12-O-tetradecanoylphorbol 13-acetate (TPA) on the activation of the CAT gene under the regulatory control of viral promoter/enhancer elements transfected into NIH-3T3, CV-1 and COS-7 cells. The results of these studies demonstrated that (1) pristane or TPA induced trans-activation of SV2cat, HIVcat, RSVcat and MMTVcat in cells transfected with each respective plasmid construct, (2) only pristane induced activation of pA10cat and pOSP/11 and (3) neither TPA nor pristane trans-activated pSV0cat. Furthermore, treatment with either pristane or TPA elicited changes in the morphology of each of the cell lines. Collectively these results indicate that pristane is a potent inducer of gene expression and exhibits similar characteristics as the tumor promoter, TPA.

KW - 12-O-tetradecanoylphorbol 13-acetate

KW - Chloramphenicol acetyltransferase

KW - Pristane

KW - Transfection

KW - trans-Activation

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ER -

Pristane induced gene activation

Abstract

Keywords

ASJC Scopus subject areas

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