TY - JOUR
T1 - Probing a hydrogen bond pair and the FAD redox properties in the proline dehydrogenase domain of Escherichia coli PutA
AU - Baban, Berevan A.
AU - Vinod, Madhavan P.
AU - Tanner, John J.
AU - Becker, Donald F.
N1 - Funding Information:
This research was supported in part by the Research (Award RI0384), ACS-PRF (36470-G4), NSF (MCB0091664), NIH grants GM61068 (Becker) and GM65546 (Tanner), University of Nebraska Biochemistry Department and Redox Biology Center, and the Nebraska Agricultural Research Division, Journal Series No. 14474. This publication was also made possible by NIH Grant Number P20 RR-017675-02 from the National Center for Research Resources. Its contents are solely the responsibility of the authors and do not necessarily represent the official views of the NIH.
PY - 2004/9/1
Y1 - 2004/9/1
N2 - The PutA flavoprotein from Escherichia coli combines DNA-binding, proline dehydrogenase (PRODH), and Δ1-pyrroline-5-carboxylate dehydrogenase (P5CDH) activities onto a single polypeptide. Recently, an X-ray crystal structure of PutA residues 87-612 was solved which identified a D370-Y540 hydrogen bond pair in the PRODH active site that appears to have an important role in shaping proline binding and the FAD redox environment. To examine the role of D370-Y540 in the PRODH active site, mutants D370A, Y540F, and D370A/Y540F were characterized in a form of PutA containing only residues 86-601 (PutA86-601) designed to mimic the known structural region of PutA (87-612). Disruption of the D370-Y540 pair only slightly diminished k cat, while more noticeable affects were observed in Km. The mutant D370A/Y540F showed the most significant changes in the pH dependence of kcat/Km and Km relative to wild-type PutA86-601 with an apparent pKa value of about 8.2 for the pH-dependent decrease in Km. From the pH profile of D370A/Y540F inhibition by L-tetrahydro-2-furoic acid (L-THFA), the pH dependency of K m in D370A/Y540F is interpreted as resulting from the deprotonation of the proline amine in the E-S complex. Replacement of D370 and Y540 produces divergent effects on the Em for bound FAD. At pH 7.0, Em values of -0.026, -0.089 and -0.042 V were determined for the two-electron reduction of bound FAD in D370A, Y540F and D370A/Y540F, respectively. The 40-mV positive shift in Em determined for D370A relative to wild-type PutA86-601 (Em=-0.066 V, pH 7.0) indicates D370 has a key role in modulating the FAD redox environment.
AB - The PutA flavoprotein from Escherichia coli combines DNA-binding, proline dehydrogenase (PRODH), and Δ1-pyrroline-5-carboxylate dehydrogenase (P5CDH) activities onto a single polypeptide. Recently, an X-ray crystal structure of PutA residues 87-612 was solved which identified a D370-Y540 hydrogen bond pair in the PRODH active site that appears to have an important role in shaping proline binding and the FAD redox environment. To examine the role of D370-Y540 in the PRODH active site, mutants D370A, Y540F, and D370A/Y540F were characterized in a form of PutA containing only residues 86-601 (PutA86-601) designed to mimic the known structural region of PutA (87-612). Disruption of the D370-Y540 pair only slightly diminished k cat, while more noticeable affects were observed in Km. The mutant D370A/Y540F showed the most significant changes in the pH dependence of kcat/Km and Km relative to wild-type PutA86-601 with an apparent pKa value of about 8.2 for the pH-dependent decrease in Km. From the pH profile of D370A/Y540F inhibition by L-tetrahydro-2-furoic acid (L-THFA), the pH dependency of K m in D370A/Y540F is interpreted as resulting from the deprotonation of the proline amine in the E-S complex. Replacement of D370 and Y540 produces divergent effects on the Em for bound FAD. At pH 7.0, Em values of -0.026, -0.089 and -0.042 V were determined for the two-electron reduction of bound FAD in D370A, Y540F and D370A/Y540F, respectively. The 40-mV positive shift in Em determined for D370A relative to wild-type PutA86-601 (Em=-0.066 V, pH 7.0) indicates D370 has a key role in modulating the FAD redox environment.
KW - Active site residue
KW - FAD
KW - FAD redox potential
KW - NAD
KW - P5CDH
KW - PRODH
KW - Proline dehydrogenase
KW - flavin adenine dinucleotide
KW - nicotinamide adenine dinucleotide
KW - proline dehydrogenase
KW - proline utilization
KW - put
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U2 - 10.1016/j.bbapap.2004.06.001
DO - 10.1016/j.bbapap.2004.06.001
M3 - Article
C2 - 15450175
AN - SCOPUS:4344640338
VL - 1701
SP - 49
EP - 59
JO - BBA - Protein Structure
JF - BBA - Protein Structure
SN - 1570-9639
IS - 1-2
ER -