TY - JOUR
T1 - Probing Ribosome Structure and Function Using Short Oligodeoxyribonucleotides
AU - Hill, Walter E.
AU - Camp, David G.
AU - Tapprich, William E.
AU - Tassanakajohn, Anchalee
N1 - Funding Information:
i This work was supported by National Institutes of Health Research Grants GM28685 and GM 19963. 2 M. Belfort, J. Pedersen-Lane, K. Ehrenman, F. K. Chu, G. F. Maley, F. Maley, D. S. McPheeters, and L. Gold, Gene 41, 93 (1986). a T. Inoue and T. R. Cech, Proc. Natl. Acad. Sci. U.S.A. 82, 648 (1985). 4 D. Moazed, S. Stern, and H. F. Noller, £ Mol. Biol. 187, 399 (1986). S. Stem, R. C. Wilson, and H. F. Noller, £ MoL BioL 192, 101 (1986).
Funding Information:
We are most grateful for the useful comments and discussions by our colleagues in various laboratories. Of special note is our appreciation to the late Dr. Gary Craven whose enthusiastic support and insight were of major importance in this work. This study was supported in part by a grant from the National Science Foundation, DMB 84-17297.
PY - 1988/1
Y1 - 1988/1
N2 - To probe the function of these regions, small complementary oligo deoxyribonucleotides (cDNA probes) have been used to hybridize specific regions of the ribosomal RNA in the ribosomal subunits after which various functions of the ribosome are checked with the probe in place. The methods used to perform these studies will be discussed in this chapter. This chapter presents various specific applications such as subunit association sites, tRNA binding sites, and other sites. That certain rRNA regions may be involved in subunit association has been suggested by several different studies. Recent evidence suggested that the 807–809 region of the 23S rRNA might base-pair with the CCA 3´-terminus of tRNA. There are many other assays that can be used to determine the function of various regions of the rRNA. Clearly, binding of mRNA can be assayed as it comes in juxtaposition with the ribosome. All in all it appears that this technique offers a way of providing substantive insight into the structure and function of regions of rRNA in the ribosome.
AB - To probe the function of these regions, small complementary oligo deoxyribonucleotides (cDNA probes) have been used to hybridize specific regions of the ribosomal RNA in the ribosomal subunits after which various functions of the ribosome are checked with the probe in place. The methods used to perform these studies will be discussed in this chapter. This chapter presents various specific applications such as subunit association sites, tRNA binding sites, and other sites. That certain rRNA regions may be involved in subunit association has been suggested by several different studies. Recent evidence suggested that the 807–809 region of the 23S rRNA might base-pair with the CCA 3´-terminus of tRNA. There are many other assays that can be used to determine the function of various regions of the rRNA. Clearly, binding of mRNA can be assayed as it comes in juxtaposition with the ribosome. All in all it appears that this technique offers a way of providing substantive insight into the structure and function of regions of rRNA in the ribosome.
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U2 - 10.1016/S0076-6879(88)64057-2
DO - 10.1016/S0076-6879(88)64057-2
M3 - Article
C2 - 3071674
AN - SCOPUS:0024272693
SN - 0076-6879
VL - 164
SP - 401
EP - 419
JO - Methods in enzymology
JF - Methods in enzymology
IS - C
ER -