Probing salt-induced and supercoiling-induced B-Z junctions in DNA by bisulfitemethoxyamine.

K. Negishi, T. Yamashita, W. Zacharias, R. D. Wells, T. Bessho, H. Hayatsu

Research output: Contribution to journalArticle

Abstract

Salt-induced and supercoiling-induced B-Z junctions in pWR756, a plasmid containing (GC)16, were probed with bisulfite-methoxyamine, a modification reagent specific for single-stranded nucleic acids. The modification sites were analyzed with S1 nuclease and the modified cytosines were determined from termination sites of DNA chain elongation by DNA polymerase. The results showed that most accessible cytosines are the same for both types of B-Z junctions.

Original languageEnglish (US)
Pages (from-to)109-110
Number of pages2
JournalNucleic acids symposium series
Issue number22
StatePublished - 1990

ASJC Scopus subject areas

  • Medicine(all)

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    Negishi, K., Yamashita, T., Zacharias, W., Wells, R. D., Bessho, T., & Hayatsu, H. (1990). Probing salt-induced and supercoiling-induced B-Z junctions in DNA by bisulfitemethoxyamine. Nucleic acids symposium series, (22), 109-110.