TY - JOUR
T1 - Probiotic bacteria change Escherichia coli-induced gene expression in cultured colonocytes
T2 - Implications in intestinal pathophysiology
AU - Panigrahi, Pinaki
AU - Braileanu, Gheorghe T.
AU - Chen, Hegang
AU - Stine, O. Colin
PY - 2007/12/21
Y1 - 2007/12/21
N2 - Aim: To investigate the change in eukaryotic gene expression profile in Caco-2 cells after infection with strains of Escherichia coli and commensal probiotic bacteria. Methods: A 19200 gene/expressed sequence tag gene chip was used to examine expression of genes alter infection of Caco-2 cells with strains of normal flora E. coli, Lactobacillus plantarum, and a combination of the two. Results: The cDNA microarray revealed up-regulation of 155 and down-regulation of 177 genes by E. coli. L. plantarum up-regulated 45 and down-regulated 36 genes. During mixed infection, 27 genes were up-regulated and 59 were down-regulated, with nullification of stimulatory/inhibitory effects on most of the genes. Expression of several new genes was noted in this group. Conclusion: The commensal bacterial strains used in this study induced the expression of a large number of genes in colonocyte-like cultured cells and changed the expression of several genes involved in important cellular processes such as regulation of transcription, protein biosynthesis, metabolism, cell adhesion, ubiquitination, and apoptosis. Such changes induced by the presence of probiotic bacteria may shape the physiologic and pathologic responses they trigger in the host.
AB - Aim: To investigate the change in eukaryotic gene expression profile in Caco-2 cells after infection with strains of Escherichia coli and commensal probiotic bacteria. Methods: A 19200 gene/expressed sequence tag gene chip was used to examine expression of genes alter infection of Caco-2 cells with strains of normal flora E. coli, Lactobacillus plantarum, and a combination of the two. Results: The cDNA microarray revealed up-regulation of 155 and down-regulation of 177 genes by E. coli. L. plantarum up-regulated 45 and down-regulated 36 genes. During mixed infection, 27 genes were up-regulated and 59 were down-regulated, with nullification of stimulatory/inhibitory effects on most of the genes. Expression of several new genes was noted in this group. Conclusion: The commensal bacterial strains used in this study induced the expression of a large number of genes in colonocyte-like cultured cells and changed the expression of several genes involved in important cellular processes such as regulation of transcription, protein biosynthesis, metabolism, cell adhesion, ubiquitination, and apoptosis. Such changes induced by the presence of probiotic bacteria may shape the physiologic and pathologic responses they trigger in the host.
KW - Escherichia coli
KW - Gene expression
KW - Lactobacillus
KW - Probiotic
KW - cDNA microarray
UR - http://www.scopus.com/inward/record.url?scp=37649013438&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=37649013438&partnerID=8YFLogxK
U2 - 10.3748/wjg.v13.i47.6370
DO - 10.3748/wjg.v13.i47.6370
M3 - Article
C2 - 18081226
AN - SCOPUS:37649013438
SN - 1007-9327
VL - 13
SP - 6370
EP - 6378
JO - World Journal of Gastroenterology
JF - World Journal of Gastroenterology
IS - 47
ER -