Probiotic bacteria change Escherichia coli-induced gene expression in cultured colonocytes: Implications in intestinal pathophysiology

Pinaki Panigrahi, Gheorghe T. Braileanu, Hegang Chen, O. Colin Stine

Research output: Contribution to journalArticlepeer-review

18 Scopus citations

Abstract

Aim: To investigate the change in eukaryotic gene expression profile in Caco-2 cells after infection with strains of Escherichia coli and commensal probiotic bacteria. Methods: A 19200 gene/expressed sequence tag gene chip was used to examine expression of genes alter infection of Caco-2 cells with strains of normal flora E. coli, Lactobacillus plantarum, and a combination of the two. Results: The cDNA microarray revealed up-regulation of 155 and down-regulation of 177 genes by E. coli. L. plantarum up-regulated 45 and down-regulated 36 genes. During mixed infection, 27 genes were up-regulated and 59 were down-regulated, with nullification of stimulatory/inhibitory effects on most of the genes. Expression of several new genes was noted in this group. Conclusion: The commensal bacterial strains used in this study induced the expression of a large number of genes in colonocyte-like cultured cells and changed the expression of several genes involved in important cellular processes such as regulation of transcription, protein biosynthesis, metabolism, cell adhesion, ubiquitination, and apoptosis. Such changes induced by the presence of probiotic bacteria may shape the physiologic and pathologic responses they trigger in the host.

Original languageEnglish (US)
Pages (from-to)6370-6378
Number of pages9
JournalWorld Journal of Gastroenterology
Volume13
Issue number47
DOIs
StatePublished - Dec 21 2007

Keywords

  • Escherichia coli
  • Gene expression
  • Lactobacillus
  • Probiotic
  • cDNA microarray

ASJC Scopus subject areas

  • Gastroenterology

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