TY - JOUR
T1 - Production and utilization of growth factors related to fibroblast growth factor by embryonal carcinoma cells and their differentiated cells
AU - Rizzino, Angie
AU - Kuszynski, Charles
AU - Ruff, Eric
AU - Tiesman, Jay
N1 - Funding Information:
Terry Riss and David Sirhasku (University of Texas Medical School, Houston, TX) are thanked for their gifts of neutralizing antibodies that recognize basic FGF and acidic FGF. Andrew Baird (Salk Institute, La Jolla, CA) is thanked for his gift of a neutralizing antibody to basic FGF. Bradley Olwin and Stephen Hauschka (University of Washington, Seattle, WA) are thanked for their gift of acidic FGF and Peter Andrews (Wistar Institute, Philadelphia, PA) is thanked for providing the NT2/Dl EC cells. Heather Rizzino is thanked for excellent editorial assistance and Ronald Hines is thanked for his helpful comments. This work was supported by grants from the National Institute of Child Health and Human Development (HD 19837) and the National Cancer Institute (Laboratory Cancer Research Center Support Grant, CA 36727). Jay Tiesman was supported by a fellowship from the Elizabeth Bruce and Parents Memorial Endowment.
PY - 1988/9
Y1 - 1988/9
N2 - Previous studies have established that embryonal carcinoma (EC) cells produce several different growth factors, but express few, if any, receptors for epidermal growth factor, platelet-derived growth factor, or transforming growth factor type-β. In this study, the production and utilization of fibroblast growth factor (FGF) by EC cells and their differentiated cells were investigated. We have determined that EC cells produce a heat-labile, heparin-binding factor that competes with FGF for binding to membrane receptors and appears to be immunologically related to FGF. The same or a similar factor is produced by three different EC cell lines, including a multipotent human EC cell line. However, production of this factor is apparently reduced when each EC cell line differentiates. Unlike the parental EC cells, the differentiated cells respond to FGF by growth stimulation and the growth responses to FGF correlate with increased binding of FGF. Although the binding data indicate that both the EC cells and their differentiated cells exhibit high affinity receptors for FGF, the differentiated cells express these receptors at levels approximately 10-fold higher. These findings suggest that the FGF-related growth factor could influence the growth of EC cells or their differentiated cells.
AB - Previous studies have established that embryonal carcinoma (EC) cells produce several different growth factors, but express few, if any, receptors for epidermal growth factor, platelet-derived growth factor, or transforming growth factor type-β. In this study, the production and utilization of fibroblast growth factor (FGF) by EC cells and their differentiated cells were investigated. We have determined that EC cells produce a heat-labile, heparin-binding factor that competes with FGF for binding to membrane receptors and appears to be immunologically related to FGF. The same or a similar factor is produced by three different EC cell lines, including a multipotent human EC cell line. However, production of this factor is apparently reduced when each EC cell line differentiates. Unlike the parental EC cells, the differentiated cells respond to FGF by growth stimulation and the growth responses to FGF correlate with increased binding of FGF. Although the binding data indicate that both the EC cells and their differentiated cells exhibit high affinity receptors for FGF, the differentiated cells express these receptors at levels approximately 10-fold higher. These findings suggest that the FGF-related growth factor could influence the growth of EC cells or their differentiated cells.
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U2 - 10.1016/0012-1606(88)90161-3
DO - 10.1016/0012-1606(88)90161-3
M3 - Article
C2 - 2842210
AN - SCOPUS:0023783022
SN - 0012-1606
VL - 129
SP - 61
EP - 71
JO - Developmental Biology
JF - Developmental Biology
IS - 1
ER -