Production of insulin-like growth factor I (IGF-I) and IGF-binding proteins by rat intestinal stromal cells in vitro

R. G. MacDonald, R. H. McCusker, D. J. Blackwood, J. A. Vanderhoof, J. H.Y. Park

Research output: Contribution to journalArticle

2 Scopus citations

Abstract

To determine if intestinal stromal cells secrete diffusible factors such as insulin-like growth factors (IGFs) capable of regulating epithelial cell growth in vitro, stromal cells were isolated by enzymatic digestion of rat intestine. Incorporation of [3H]thymidine into DNA and [14C]leucine into protein of IEC-6 cells, a model intestinal epithelial cell line, was significantly increased (two- to threefold) when the IEC-6 cells were co-cultured with stromal cells, relative to IEC-6 cells grown alone. Medium conditioned by stromal cells stimulated DNA synthesis of IEC-6 cells in a dose-dependent manner. Analysis of the conditioned medium revealed that intestinal stromal cells secreted IGF-I, but little IGF-II, in addition to an M(r) 32,000 IGF-binding protein (IGFBP-2) and an IGFBP having M(r) ~ 24,000. We conclude that rat intestinal stromal cells secrete one or more diffusible factors, which may include IGF-I and IGFBPs, capable of stimulating proliferation of IEC-6 cells in vitro.

Original languageEnglish (US)
Pages (from-to)158-166
Number of pages9
JournalCellular and Molecular Life Sciences
Volume54
Issue number2
DOIs
StatePublished - Apr 7 1998

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Keywords

  • Epithelium
  • IGFs
  • Igfbp
  • Insulin-like growth factors
  • Stroma
  • Stromal-epithelial interactions

ASJC Scopus subject areas

  • Molecular Medicine
  • Molecular Biology
  • Pharmacology
  • Cellular and Molecular Neuroscience
  • Cell Biology

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