Progress toward characterization of the group A Streptococcus metagenome: Complete genome sequence of a macrolide-resistant serotype M6 strain

David J. Banks; Stephen F. Porcella; Kent D. Barbian; Stephen B. Beres; Lauren E. Philips; Jovanka M. Voyich; Frank R. DeLeo; Judith M. Martin; Greg A. Somerville; James M. Musser

doi:10.1086/422697

Progress toward characterization of the group A Streptococcus metagenome: Complete genome sequence of a macrolide-resistant serotype M6 strain

David J. Banks, Stephen F. Porcella, Kent D. Barbian, Stephen B. Beres, Lauren E. Philips, Jovanka M. Voyich, Frank R. DeLeo, Judith M. Martin, Greg A. Somerville, James M. Musser

Research output: Contribution to journal › Article › peer-review

150 Scopus citations

Abstract

We describe the genome sequence of a macrolide-resistant strain (MGAS10394) of serotype M6 group A Streptococcus (GAS). The genome is 1,900,156 bp in length, and 8 prophage-like elements or remnants compose 12.4% of the chromosome. A 8.3-kb prophage remnant encodes the SpeA4 variant of streptococcal pyrogenic exotoxin A. The genome of strain MGAS10394 contains a chimeric genetic element composed of prophage genes and a transposon encoding the mefA gene conferring macrolide resistance. This chimeric element also has a gene encoding a novel surface-exposed protein (designated "R6 protein"), with an LPKTG cell-anchor motif located at the carboxyterminus. Surface expression of this protein was confirmed by flow cytometry. Humans with GAS pharyngitis caused by serotype M6 strains had antibody against the R6 protein present in convalescent, but not acute, serum samples. Our studies add to the theme that GAS prophage-encoded extracellular proteins contribute to host-pathogen interactions in a strain-specific fashion.

Original language	English (US)
Pages (from-to)	727-738
Number of pages	12
Journal	Journal of Infectious Diseases
Volume	190
Issue number	4
DOIs	https://doi.org/10.1086/422697
State	Published - Aug 15 2004
Externally published	Yes

ASJC Scopus subject areas

Immunology and Allergy
Infectious Diseases

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Banks, D. J., Porcella, S. F., Barbian, K. D., Beres, S. B., Philips, L. E., Voyich, J. M., DeLeo, F. R., Martin, J. M., Somerville, G. A., & Musser, J. M. (2004). Progress toward characterization of the group A Streptococcus metagenome: Complete genome sequence of a macrolide-resistant serotype M6 strain. Journal of Infectious Diseases, 190(4), 727-738. https://doi.org/10.1086/422697

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title = "Progress toward characterization of the group A Streptococcus metagenome: Complete genome sequence of a macrolide-resistant serotype M6 strain",

abstract = "We describe the genome sequence of a macrolide-resistant strain (MGAS10394) of serotype M6 group A Streptococcus (GAS). The genome is 1,900,156 bp in length, and 8 prophage-like elements or remnants compose 12.4% of the chromosome. A 8.3-kb prophage remnant encodes the SpeA4 variant of streptococcal pyrogenic exotoxin A. The genome of strain MGAS10394 contains a chimeric genetic element composed of prophage genes and a transposon encoding the mefA gene conferring macrolide resistance. This chimeric element also has a gene encoding a novel surface-exposed protein (designated {"}R6 protein{"}), with an LPKTG cell-anchor motif located at the carboxyterminus. Surface expression of this protein was confirmed by flow cytometry. Humans with GAS pharyngitis caused by serotype M6 strains had antibody against the R6 protein present in convalescent, but not acute, serum samples. Our studies add to the theme that GAS prophage-encoded extracellular proteins contribute to host-pathogen interactions in a strain-specific fashion.",

author = "Banks, {David J.} and Porcella, {Stephen F.} and Barbian, {Kent D.} and Beres, {Stephen B.} and Philips, {Lauren E.} and Voyich, {Jovanka M.} and DeLeo, {Frank R.} and Martin, {Judith M.} and Somerville, {Greg A.} and Musser, {James M.}",

year = "2004",

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AU - Beres, Stephen B.

AU - Philips, Lauren E.

AU - Voyich, Jovanka M.

AU - DeLeo, Frank R.

AU - Martin, Judith M.

AU - Somerville, Greg A.

AU - Musser, James M.

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N2 - We describe the genome sequence of a macrolide-resistant strain (MGAS10394) of serotype M6 group A Streptococcus (GAS). The genome is 1,900,156 bp in length, and 8 prophage-like elements or remnants compose 12.4% of the chromosome. A 8.3-kb prophage remnant encodes the SpeA4 variant of streptococcal pyrogenic exotoxin A. The genome of strain MGAS10394 contains a chimeric genetic element composed of prophage genes and a transposon encoding the mefA gene conferring macrolide resistance. This chimeric element also has a gene encoding a novel surface-exposed protein (designated "R6 protein"), with an LPKTG cell-anchor motif located at the carboxyterminus. Surface expression of this protein was confirmed by flow cytometry. Humans with GAS pharyngitis caused by serotype M6 strains had antibody against the R6 protein present in convalescent, but not acute, serum samples. Our studies add to the theme that GAS prophage-encoded extracellular proteins contribute to host-pathogen interactions in a strain-specific fashion.

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Progress toward characterization of the group A Streptococcus metagenome: Complete genome sequence of a macrolide-resistant serotype M6 strain

Abstract

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