Proliferation of wound derived capillary endothelial cells: young versus aged

Gregg D. Phillips; A. Marika Stone; Julie C. Schultz; Bryan D. Jones; David R. Knighton

doi:10.1016/0047-6374(94)90021-3

Proliferation of wound derived capillary endothelial cells: young versus aged

Gregg D. Phillips, A. Marika Stone, Julie C. Schultz, Bryan D. Jones, David R. Knighton

Research output: Contribution to journal › Article › peer-review

13 Scopus citations

Abstract

The objective of this study was to compare the proliferative potential of wound derived capillary endothelial cells (WCEC) from aged and young rats. Endothelial cells were isolated from subcutaneously implanted sponges in 2- and 24-month-old rats. The identity of the cells as endothelial was confirmed by staining for Ac-LDL uptake. Aged and young WCEC (20 000/well) were stimulated with increasing concentrations of fetal calf serum (0, 2.5, 5, 10 and 15%). The increase in cell number was determined with a Coulter counter. At all serum concentrations, the proliferative capacity of WCEC from aged rats was significantly higher than that of WCEC from young rats.

Original language	English (US)
Pages (from-to)	141-148
Number of pages	8
Journal	Mechanisms of Ageing and Development
Volume	77
Issue number	2
DOIs	https://doi.org/10.1016/0047-6374(94)90021-3
State	Published - Dec 16 1994
Externally published	Yes

Keywords

Aging
Angiogenesis
Endothelial cells
Proliferation
Wound healing

ASJC Scopus subject areas

Aging
Developmental Biology

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10.1016/0047-6374(94)90021-3

Cite this

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title = "Proliferation of wound derived capillary endothelial cells: young versus aged",

abstract = "The objective of this study was to compare the proliferative potential of wound derived capillary endothelial cells (WCEC) from aged and young rats. Endothelial cells were isolated from subcutaneously implanted sponges in 2- and 24-month-old rats. The identity of the cells as endothelial was confirmed by staining for Ac-LDL uptake. Aged and young WCEC (20 000/well) were stimulated with increasing concentrations of fetal calf serum (0, 2.5, 5, 10 and 15%). The increase in cell number was determined with a Coulter counter. At all serum concentrations, the proliferative capacity of WCEC from aged rats was significantly higher than that of WCEC from young rats.",

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T2 - young versus aged

AU - Phillips, Gregg D.

AU - Marika Stone, A.

AU - Schultz, Julie C.

AU - Jones, Bryan D.

AU - Knighton, David R.

PY - 1994/12/16

Y1 - 1994/12/16

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AB - The objective of this study was to compare the proliferative potential of wound derived capillary endothelial cells (WCEC) from aged and young rats. Endothelial cells were isolated from subcutaneously implanted sponges in 2- and 24-month-old rats. The identity of the cells as endothelial was confirmed by staining for Ac-LDL uptake. Aged and young WCEC (20 000/well) were stimulated with increasing concentrations of fetal calf serum (0, 2.5, 5, 10 and 15%). The increase in cell number was determined with a Coulter counter. At all serum concentrations, the proliferative capacity of WCEC from aged rats was significantly higher than that of WCEC from young rats.

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KW - Angiogenesis

KW - Endothelial cells

KW - Proliferation

KW - Wound healing

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Proliferation of wound derived capillary endothelial cells: young versus aged

Abstract

Keywords

ASJC Scopus subject areas

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