Proliferation of wound derived capillary endothelial cells: young versus aged

Gregg D. Phillips, A. Marika Stone, Julie C. Schultz, Bryan D. Jones, David R. Knighton

Research output: Contribution to journalArticlepeer-review

12 Scopus citations

Abstract

The objective of this study was to compare the proliferative potential of wound derived capillary endothelial cells (WCEC) from aged and young rats. Endothelial cells were isolated from subcutaneously implanted sponges in 2- and 24-month-old rats. The identity of the cells as endothelial was confirmed by staining for Ac-LDL uptake. Aged and young WCEC (20 000/well) were stimulated with increasing concentrations of fetal calf serum (0, 2.5, 5, 10 and 15%). The increase in cell number was determined with a Coulter counter. At all serum concentrations, the proliferative capacity of WCEC from aged rats was significantly higher than that of WCEC from young rats.

Original languageEnglish (US)
Pages (from-to)141-148
Number of pages8
JournalMechanisms of Ageing and Development
Volume77
Issue number2
DOIs
StatePublished - Dec 16 1994

Keywords

  • Aging
  • Angiogenesis
  • Endothelial cells
  • Proliferation
  • Wound healing

ASJC Scopus subject areas

  • Aging
  • Developmental Biology

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