Promise of Combining Antifungal Agents in Denture Adhesives to Fight Candida Species Infections

Jorge L. Garaicoa, Carol L. Fischer, Amber M. Bates, Julie Holloway, Gustavo Avila-Ortiz, Janet M. Guthmiller, Georgia K. Johnson, Clark Stanford, Kim A. Brogden

Research output: Contribution to journalArticle

6 Scopus citations

Abstract

Purpose: Several complications may arise in patients wearing complete prosthetic appliances, including denture-associated infections and mucosal stomatitis due to Candida species. This study evaluated the activity of anti-Candida agents in denture adhesive and the cytotoxicities of these preparations for primary human gingival epithelial (GE) keratinocytes. Materials and Methods: The anti-Candida activities of antimicrobial peptides, antimicrobial lipids, and antifungal agents against C. albicans ATCC 64124 or HMV4C were assessed in microdilution assays containing water or 1% denture adhesive. The minimal inhibitory concentrations (MIC) and the minimal bactericidal concentrations (MBC) were determined. The cytotoxicities of denture adhesive compounded with these agents were assessed in 1.0 × 105 primary GE keratinocytes in LGM-3 media with resazurin. Results: Lactoferricin B, SMAP28, sphingosine, dihydrosphingosine, and phytosphingosine in 1% denture adhesive lost antimicrobial activity for C. albicans (p < 0.05). Amphotericin B, chlorhexidine dihydrochloride, chlorhexidine gluconate, fluconazole, and nystatin in 1% denture adhesive or compounded directly into denture adhesive and then diluted to 1% adhesive, did not lose antimicrobial activity. Compounded formulations were not cytotoxic (LD50 > 100.0 μg/ml) against primary human GE keratinocytes. Conclusions: Antimicrobial peptides and antimicrobial lipids had diminished activities in 1% adhesive, suggesting that components in adhesives may inactivate local innate immune factors in the oral cavity, possibly predisposing denture wearers to Candida species infections. More importantly, antifungal agents retained their anti-C. albicans activities in denture adhesive, strongly suggesting that antifungal agents could be candidates for inclusion in adhesive formulations and used as prescribed topical treatments for individuals with denture stomatitis.

Original languageEnglish (US)
Pages (from-to)755-762
Number of pages8
JournalJournal of Prosthodontics
Volume27
Issue number8
DOIs
StatePublished - Oct 2018

Keywords

  • Antimicrobial peptides
  • Candida
  • antifungal agents
  • antimicrobial lipids
  • denture adhesive
  • denture prosthesis
  • keratinocyte
  • stomatitis

ASJC Scopus subject areas

  • Dentistry(all)

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  • Cite this

    Garaicoa, J. L., Fischer, C. L., Bates, A. M., Holloway, J., Avila-Ortiz, G., Guthmiller, J. M., Johnson, G. K., Stanford, C., & Brogden, K. A. (2018). Promise of Combining Antifungal Agents in Denture Adhesives to Fight Candida Species Infections. Journal of Prosthodontics, 27(8), 755-762. https://doi.org/10.1111/jopr.12565