Promoting apoptosis: A novel activity associated with the cyclin- dependent kinase inhibitor p27

Yu Katayose, Min Kim, Amol N.S. Rakkar, Zhuangwu Li, Kenneth H. Cowan, Prem Seth

Research output: Contribution to journalArticlepeer-review

326 Scopus citations

Abstract

p27(Kip1), a cyclin-dependent kinase inhibitor, is recognized as a negative regulator of the cell cycle. In this paper, we report that overexpression of p27(Kip1) triggers apoptosis in several different human cancer cell lines. Using a recombinant adenoviral vector that expresses p27(Kip1) (Adp27), we found that overexpression of p27(Kip1) in MDA-MB-231 breast cancer cells induces apoptosis that was seen by a number of different techniques, including flow cytometry and in situ terminal deoxynucleotidyl transferase-mediated nick end labeling, flow cytometric assay for sub-G1 population, and 4',6-diamindino-2-phenylindole staining. Cleavage of poly(ADP-ribose) polymerase and degradation of cyclin B1, events that are known to be associated with apoptosis, were also observed following overexpression of p27(Kip1). This is the first report indicating a role for p27(Kip1) in induction of apoptosis.

Original languageEnglish (US)
Pages (from-to)5441-5445
Number of pages5
JournalCancer Research
Volume57
Issue number24
StatePublished - Dec 15 1997
Externally publishedYes

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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