p27(Kip1), a cyclin-dependent kinase inhibitor, is recognized as a negative regulator of the cell cycle. In this paper, we report that overexpression of p27(Kip1) triggers apoptosis in several different human cancer cell lines. Using a recombinant adenoviral vector that expresses p27(Kip1) (Adp27), we found that overexpression of p27(Kip1) in MDA-MB-231 breast cancer cells induces apoptosis that was seen by a number of different techniques, including flow cytometry and in situ terminal deoxynucleotidyl transferase-mediated nick end labeling, flow cytometric assay for sub-G1 population, and 4',6-diamindino-2-phenylindole staining. Cleavage of poly(ADP-ribose) polymerase and degradation of cyclin B1, events that are known to be associated with apoptosis, were also observed following overexpression of p27(Kip1). This is the first report indicating a role for p27(Kip1) in induction of apoptosis.
|Original language||English (US)|
|Number of pages||5|
|State||Published - Dec 15 1997|
ASJC Scopus subject areas
- Cancer Research