Abstract
The effects of vasoactive intestinal polypeptide (VIP) and dibutyryl cyclic adenosine 3′:5′monophosphate (dbcAMP) on two human colon carcinoma cell lines, HCT 116 and GEO, were investigated. VIP and dbcAMP inhibited the growth of both cell lines in monolayer culture in a dose-dependent manner. Within 6 h of treatment with 1 mM dbcAMP or 0.3 μM VIP, numerous mucin-like droplets were secreted by GEO cells. VIP and dbcAMP also increased carcinoembryonic antigen (CEA) secretion. In both cell lines, a 9-fold increase in conditioned medium CEA levels was observed at 1 mM dbcAMP and a 2.6-fold increase at 1.5 μM VIP. Time- and concentrationdependent evaluation in cAMP levels were elicited by VIP in the two cell lines. Immunocytochemical studies for cell-surface glycoprotein detection in GEO cells showed that VIP induced a morphological and functional organization of mucin-secreting cells. These results indicate that VIP and dbcAMP have antiproliferative and strong differentiation-promoting effects in colon cancer cells. This is the first report of VIP-induced mucin secretion in colon tumor cells.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 15-26 |
| Number of pages | 12 |
| Journal | Regulatory Peptides |
| Volume | 24 |
| Issue number | 1 |
| DOIs | |
| State | Published - Jan 1989 |
| Externally published | Yes |
Keywords
- Adenylate cyclase
- Antiproliferative effect
- Carcinoembryonic antigen
- Dibutyril cyclic AMP
- Mucin secretion
ASJC Scopus subject areas
- Biochemistry
- Physiology
- Endocrinology
- Clinical Biochemistry
- Cellular and Molecular Neuroscience