TY - JOUR
T1 - Proopiomelanocortin-derived peptides, phosphoinositides, cAMP, and aldosterone secretion
AU - Ganguly, Arunabha
AU - Chiou, Shirley
AU - West, Leigh A.
AU - Davis, John S.
PY - 1989/12
Y1 - 1989/12
N2 - Since the intracellular messengers of various proopiomelanocortin-derived peptides remain ambiguous at best, we have investigated the possible involvement of phosphoinositide metabolism in aldosterone secretion evoked by α-MSH, β-LPH, as well as ACTH in rat and calf adrenal glomerulosa cells. We have also examined the cAMP responses in the adrenal glomerulosa cells to α-MSH comparing it with those of ACTH. Our results showed that neither α-MSH, β-LPH, nor ACTH increased inositol triphosphate (IP3) or other inositol phosphates in adrenal glomerulosa cells while increasing aldosterone secretion from the same cells. Angiotensin II, known to cause hydrolysis of the phosphoinositides, increased IP3 in these adrenal cells in a dose-dependent manner. Both ACTH and α-MSH raised the cAMP levels in the calf adrenal glomerulosa cells, although the magnitude of the increase of cAMP in response to ACTH was greater. These findings suggest that IP3 as a mediator of α-MSH-and β-LPH-induced aldosterone secretion is not likely and other mediator(s) may be involved.
AB - Since the intracellular messengers of various proopiomelanocortin-derived peptides remain ambiguous at best, we have investigated the possible involvement of phosphoinositide metabolism in aldosterone secretion evoked by α-MSH, β-LPH, as well as ACTH in rat and calf adrenal glomerulosa cells. We have also examined the cAMP responses in the adrenal glomerulosa cells to α-MSH comparing it with those of ACTH. Our results showed that neither α-MSH, β-LPH, nor ACTH increased inositol triphosphate (IP3) or other inositol phosphates in adrenal glomerulosa cells while increasing aldosterone secretion from the same cells. Angiotensin II, known to cause hydrolysis of the phosphoinositides, increased IP3 in these adrenal cells in a dose-dependent manner. Both ACTH and α-MSH raised the cAMP levels in the calf adrenal glomerulosa cells, although the magnitude of the increase of cAMP in response to ACTH was greater. These findings suggest that IP3 as a mediator of α-MSH-and β-LPH-induced aldosterone secretion is not likely and other mediator(s) may be involved.
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U2 - 10.1016/0022-4731(89)90422-6
DO - 10.1016/0022-4731(89)90422-6
M3 - Article
C2 - 2559249
AN - SCOPUS:0024784506
VL - 33
SP - 1143
EP - 1148
JO - Journal of Steroid Biochemistry and Molecular Biology
JF - Journal of Steroid Biochemistry and Molecular Biology
SN - 0960-0760
IS - 6
ER -