Properties of marmoset T lymphocytes cultured in vitro

D. R. Johnson, L. Falk, R. Yang, F. Deinhardt

Research output: Contribution to journalArticle

Abstract

Marmoset splenic or circulating lymphocytes can be transformed in vitro with Herpesvirus ateles (HVA); the transformed lymphocytes grow as continuous lymphoblastoid cell cultures and can be cultivated indefinitely. These transformed cells possessed properties of T lymphocytes: 30-70% of cells formed E rosettes, all lacked surface Ig but reacted with a specific antimarmoset T lymphocyte serum. Cells of 10 lymphoblastoid cultures, derived after transformation in vitro of marmoset circulating lymphocytes, were analyzed for 5 surface characteristics: anti T lymphocyte serum reacted in a 51Cr assay with cells of all 10 cultures at 42-86% cytotoxicity (this serum reacts only slightly with cultured B lymphocytes). Cells of 5 lines formed E rosettes (11-65% reactive cells) and EAC rosettes (18-55% reactive cells) and cells of the other 5 cultures formed neither E or EAC rosettes. None of the cells possessed detectable surface Ig, however 5-10% of cells were positive for Fc receptors. These results show that cultured lymphoblastoid cells express T and/or B markers, however E or EAC rosette formation is not consistent criteria for classification of lymphocytes and therefore other markers must be determined. In contrast to lymphocytes transformed with Epstein Barr virus that are always B lymphocytes and express B lymphocyte characteristics, HVA appears to transform T lymphocytes which express variable characteristics.

Original languageEnglish (US)
Pages (from-to)No. 4391
JournalFederation Proceedings
Volume34
Issue number3
StatePublished - 1975

ASJC Scopus subject areas

  • Medicine(all)

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    Johnson, D. R., Falk, L., Yang, R., & Deinhardt, F. (1975). Properties of marmoset T lymphocytes cultured in vitro. Federation Proceedings, 34(3), No. 4391.