Marmoset splenic or circulating lymphocytes can be transformed in vitro with Herpesvirus ateles (HVA); the transformed lymphocytes grow as continuous lymphoblastoid cell cultures and can be cultivated indefinitely. These transformed cells possessed properties of T lymphocytes: 30-70% of cells formed E rosettes, all lacked surface Ig but reacted with a specific antimarmoset T lymphocyte serum. Cells of 10 lymphoblastoid cultures, derived after transformation in vitro of marmoset circulating lymphocytes, were analyzed for 5 surface characteristics: anti T lymphocyte serum reacted in a 51Cr assay with cells of all 10 cultures at 42-86% cytotoxicity (this serum reacts only slightly with cultured B lymphocytes). Cells of 5 lines formed E rosettes (11-65% reactive cells) and EAC rosettes (18-55% reactive cells) and cells of the other 5 cultures formed neither E or EAC rosettes. None of the cells possessed detectable surface Ig, however 5-10% of cells were positive for Fc receptors. These results show that cultured lymphoblastoid cells express T and/or B markers, however E or EAC rosette formation is not consistent criteria for classification of lymphocytes and therefore other markers must be determined. In contrast to lymphocytes transformed with Epstein Barr virus that are always B lymphocytes and express B lymphocyte characteristics, HVA appears to transform T lymphocytes which express variable characteristics.
|Original language||English (US)|
|Pages (from-to)||No. 4391|
|State||Published - 1975|
ASJC Scopus subject areas