Hormones detected in environments have aroused wide public concern due to their possible adverse effects to humans and ecosystems. Currently, information is still very limited on the fate and transport of hormones in the soil environment. This chapter describes the methodology of fractionating soil particles into different size fractions, how to evaluate soil particles' sorption-desorption properties for testosterone, and how to link the mass and particle size distribution of the soil with the hormone's transport to surface or ground water. Results show small particles play dominant role in facilitating the transport of hormones due to their high sorption capacity, low desorption potential and easier mobility through runoff. These results provide indirect evidence on the colloids (clay)-facilitated transport of hormones to surface water and to groundwater via preferential flow. Soil particles may be fractionated into a fully-or non-dispersed size distribution. As a future research direction, a new protocol concerning the effects and roles of non-dispersed soil particles on the fate and transport of micro-pollutants should be set up with the corresponding procedures being standardized.