TY - JOUR
T1 - Propofol induces ERK-dependant expression of c-Fos and Egr-1 in neuronal cells
AU - Kidambi, Srivatsan
AU - Yarmush, Joel
AU - Fong, Wayne
AU - Kamath, Sangeetha
AU - Schianodicola, Joseph
AU - Nahmias, Yaakov
PY - 2009/5/6
Y1 - 2009/5/6
N2 - This study explored the effects of propofol on c-Fos and Egr-1 in neuroblastoma (N2A) cells. We demonstrate that propofol induced the expression of c-Fos and Egr-1 within 30 and 60min of exposure time. At 16.8μM concentration, propofol induced a 6 and 2.5-fold expression of c-Fos and Egr-1, respectively. However, at concentrations above 100μM, propofol failed to induce expression of c-Fos or Egr-1. Propofol-induced c-Fos and Egr-1 transcription was unaffected by bicuculline, a γ-aminobutyric acid-A receptor antagonist, but was abolished by PD98059, a mitogen-activated protein kinase/extracellular signal-regulated kinase inhibitor. Our study shows that clinically relevant concentrations of propofol induce c-Fos and Egr-1 expression through an extracellular signal-regulated kinase mediated and γ-aminobutyric acid-A independent pathway.
AB - This study explored the effects of propofol on c-Fos and Egr-1 in neuroblastoma (N2A) cells. We demonstrate that propofol induced the expression of c-Fos and Egr-1 within 30 and 60min of exposure time. At 16.8μM concentration, propofol induced a 6 and 2.5-fold expression of c-Fos and Egr-1, respectively. However, at concentrations above 100μM, propofol failed to induce expression of c-Fos or Egr-1. Propofol-induced c-Fos and Egr-1 transcription was unaffected by bicuculline, a γ-aminobutyric acid-A receptor antagonist, but was abolished by PD98059, a mitogen-activated protein kinase/extracellular signal-regulated kinase inhibitor. Our study shows that clinically relevant concentrations of propofol induce c-Fos and Egr-1 expression through an extracellular signal-regulated kinase mediated and γ-aminobutyric acid-A independent pathway.
KW - Extracellular signal-regulated kinase
KW - Immediate early genes
KW - Propofol
KW - γ-aminobutyric acid
UR - http://www.scopus.com/inward/record.url?scp=67049171035&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=67049171035&partnerID=8YFLogxK
U2 - 10.1097/WNR.0b013e328329a449
DO - 10.1097/WNR.0b013e328329a449
M3 - Article
C2 - 19349923
AN - SCOPUS:67049171035
SN - 0959-4965
VL - 20
SP - 657
EP - 662
JO - NeuroReport
JF - NeuroReport
IS - 7
ER -