Propofol induces ERK-dependant expression of c-Fos and Egr-1 in neuronal cells

Srivatsan Kidambi, Joel Yarmush, Wayne Fong, Sangeetha Kamath, Joseph Schianodicola, Yaakov Nahmias

Research output: Contribution to journalArticle

6 Scopus citations

Abstract

This study explored the effects of propofol on c-Fos and Egr-1 in neuroblastoma (N2A) cells. We demonstrate that propofol induced the expression of c-Fos and Egr-1 within 30 and 60min of exposure time. At 16.8μM concentration, propofol induced a 6 and 2.5-fold expression of c-Fos and Egr-1, respectively. However, at concentrations above 100μM, propofol failed to induce expression of c-Fos or Egr-1. Propofol-induced c-Fos and Egr-1 transcription was unaffected by bicuculline, a γ-aminobutyric acid-A receptor antagonist, but was abolished by PD98059, a mitogen-activated protein kinase/extracellular signal-regulated kinase inhibitor. Our study shows that clinically relevant concentrations of propofol induce c-Fos and Egr-1 expression through an extracellular signal-regulated kinase mediated and γ-aminobutyric acid-A independent pathway.

Original languageEnglish (US)
Pages (from-to)657-662
Number of pages6
JournalNeuroReport
Volume20
Issue number7
DOIs
StatePublished - May 6 2009

Keywords

  • Extracellular signal-regulated kinase
  • Immediate early genes
  • Propofol
  • γ-aminobutyric acid

ASJC Scopus subject areas

  • Neuroscience(all)

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    Kidambi, S., Yarmush, J., Fong, W., Kamath, S., Schianodicola, J., & Nahmias, Y. (2009). Propofol induces ERK-dependant expression of c-Fos and Egr-1 in neuronal cells. NeuroReport, 20(7), 657-662. https://doi.org/10.1097/WNR.0b013e328329a449