Prostaglandin F induces expression of activating transcription factor 3 (ATF3) and activates MAPK signaling in the rat corpus luteum

Nannan Guo, Chenling Meng, Wujiao Bai, Quanwei Wei, Fangxiong Shi, John S. Davis, Dagan Mao

Research output: Contribution to journalArticlepeer-review

9 Scopus citations


The current study was conducted to evaluate the expression of ATF3, in association with the activation of mitogen-activated protein kinases (MAPK) during prostaglandin F analog (PGF)-induced luteal regression in rats. A sequential PMSG/hCG treatment paradigm was used to obtain a single, well-defined generation of corpora lutea (CL) in rats. Rats were treated with PGF for 0-4h on day 7 of pseudopregnancy. Results showed that serum progesterone (P4) concentrations declined in a time dependent manner. Western blot results revealed that ATF3 increased within 2h post-PGF injection. Phosphorylated ERK1/2 (p-ERK) and JNK (p-JNK) increased within 30min and then were gradually reduced in response to PGF. In contrast, the levels of phosphorylated p38 MAPK (p-p38) were not significantly altered. The immunostaining density for p-ERK decreased from the periphery to the center of the corpus luteum following treatment with PGF, while ATF3 was expressed uniformly in the nuclei of luteal steroidogenic cells. These results indicated that treatment with PGF in vivo could induce increases in MAPK phosphorylation, especially in p-ERK, which might be correlated with the increases in ATF3 expression and the decline in P4 concentrations. To our knowledge, this is the first study to provide evidence for temporal relationships between MAPK activation and ATF3 expression during PGF-induced luteal regression in the rat.

Original languageEnglish (US)
Pages (from-to)211-218
Number of pages8
JournalActa Histochemica
Issue number2
StatePublished - Mar 1 2015


  • ATF3
  • CL
  • MAPK
  • P
  • PGF
  • Rat

ASJC Scopus subject areas

  • Histology
  • Cell Biology


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