Protection of two PAI-2 mutants against TNF-α-mediated apoptosis

Yu Qing Zhang, Min Hou, Xia Wang, Xing Hui Sun, Ping Li, Yun Song Zhu

Research output: Contribution to journalArticlepeer-review

Abstract

Purpose: To investigate different protective activities of two PAI-2 mutants against TNF-α mediated apopotosis and to explore the effect of the spatial structure of CD interhelical region of PAI-2 on its anti-apoptotic activity. Methods: Two PAI-2 mutants, PAI-2D and PAI-2M, were cloned into eukaryotic expression vector respectively and were transfected into Hela cells. After being analyzed by RT-PCR, the positive cell strains were obtained. The anti-apoptotic effects of these two mutants were compared by MTT assay and computer-aided homology modeling revealed some changes of their spatial structures. Results: PAI-2M could protect cells from the cytotoxic effects of TNF-α, while PAI-2D lost its protection function. Mostly PAI-2M retained the spatial structure of wild type PAI-2, while PAI-2D showed obvious difference, especially in the CD interhelical region. Conclusions: The protection of PAI-2 from TNF-α induced apoptosis depends on the spatial integrality of CD interhelical region of PAI-2, and the PENF domain (PENF 73-76in the CD interhelical region is not so important that it could be substituted.

Original languageEnglish (US)
Pages (from-to)313-316
Number of pages4
JournalFudan University Journal of Medical Sciences
Volume30
Issue number4
StatePublished - Jul 2003
Externally publishedYes

Keywords

  • Apoptisis
  • Mutant
  • Plasminogen activator inhibitor type-2
  • Tumor necrosis factur-α

ASJC Scopus subject areas

  • Medicine(all)

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