Abstract
Purpose: To investigate different protective activities of two PAI-2 mutants against TNF-α mediated apopotosis and to explore the effect of the spatial structure of CD interhelical region of PAI-2 on its anti-apoptotic activity. Methods: Two PAI-2 mutants, PAI-2D and PAI-2M, were cloned into eukaryotic expression vector respectively and were transfected into Hela cells. After being analyzed by RT-PCR, the positive cell strains were obtained. The anti-apoptotic effects of these two mutants were compared by MTT assay and computer-aided homology modeling revealed some changes of their spatial structures. Results: PAI-2M could protect cells from the cytotoxic effects of TNF-α, while PAI-2D lost its protection function. Mostly PAI-2M retained the spatial structure of wild type PAI-2, while PAI-2D showed obvious difference, especially in the CD interhelical region. Conclusions: The protection of PAI-2 from TNF-α induced apoptosis depends on the spatial integrality of CD interhelical region of PAI-2, and the PENF domain (PENF 73-76in the CD interhelical region is not so important that it could be substituted.
Original language | English (US) |
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Pages (from-to) | 313-316 |
Number of pages | 4 |
Journal | Fudan University Journal of Medical Sciences |
Volume | 30 |
Issue number | 4 |
State | Published - Jul 2003 |
Externally published | Yes |
Keywords
- Apoptisis
- Mutant
- Plasminogen activator inhibitor type-2
- Tumor necrosis factur-α
ASJC Scopus subject areas
- General Medicine