Protein expression profiling of lens epithelial cells from Prdx6-depleted mice and their vulnerability to UV radiation exposure

Eri Kubo, Nailia Hasanova, Yukie Tanaka, Nigar Fatma, Yoshihiro Takamura, Dhirendra P. Singh, Yoshio Akagi

Research output: Contribution to journalArticle

30 Scopus citations

Abstract

Oxidative stress is one of the causative factors in progression and etiology of agerelated cataract. Peroxiredoxin 6 (Prdx6), a savior for cells from internal or external environmental stresses, plays a role in cellular signaling by detoxifying reactive oxygen species (ROS) and thereby controlling gene regulation. Using targeted inactivation of the Prdx6 gene, we show that Prdx6-deficient lens epithelial cells (LECs) are more vulnerable to UV-triggered cell death, a major cause of skin disorders including cataractogenesis, and these cells display abnormal protein profiles. PRDX6-depleted LECs showed phenotypic changes and formed lentoid body, a characteristic of terminal cell differentiation and epithelial-mesenchymal transition. Prdx6-/- LECs exposed to UV-B showed higher ROS expression and were prone to apoptosis compared with wild-type LECs, underscoring a protective role for Prdx6. Comparative proteomic analysis using fluorescence-based difference gel electrophoresis along with mass spectrometry and database searching revealed a total of 13 proteins that were differentially expressed in Prdx6-/- cells. Six proteins were upregulated, whereas expression of seven proteins was decreased compared with Prdx6+/+ LECs. Among the cytoskeleton- associated proteins that were highly expressed in Prdx6-deficient LECs was tropomyosin (Tm)2β. Protein blot and real-time PCR validated dramatic increase of Tm2β and Tm1α expression in these cells. Importantly, Prdx6+/+ LECs showed a similar pattern of Tm2β protein expression after transforming growth factor (TGF)-β or H2O 2 treatment. An extrinsic supply of PRDX6 could restore Tm2β expression, demonstrating that PRDX6 may attenuate adverse signaling in cells and thereby maintain cellular homeostasis. Exploring redox-proteomics (Prdx6-/-) and characterization and identification of abnormally expressed proteins and their attenuation by PRDX6 delivery should provide a basis for development of novel therapeutic interventions to postpone ROS-mediated abnormal signaling deleterious to cells or tissues.

Original languageEnglish (US)
Pages (from-to)C342-C354
JournalAmerican Journal of Physiology - Cell Physiology
Volume298
Issue number2
DOIs
StatePublished - Feb 2010

Keywords

  • Oxidative stress
  • Peroxiredoxin
  • Proteomics
  • Tropomyosin

ASJC Scopus subject areas

  • Physiology
  • Cell Biology

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