Protein kinase C pathway is involved in regulating the secretion of prostatic acid phosphatase in human prostate cancer cells

Ming Fong Lin, Xiu Qing Zhang, Jeanenne Dean, Fen Fen Lin

Research output: Contribution to journalArticlepeer-review

7 Scopus citations

Abstract

The stimulated secretion of prostatic acid phosphatase (PAcP) has been known to be a hallmark of androgen action on human prostate epithelial cells for the last five decades. The molecular mechanism of androgen action on PAcP secretion, however, has remained mostly unknown. We investigated the molecular mechanism that promotes PAcP secretion in LNCaP human prostate carcinoma cells which express PAcP and are androgen-responsive. Treatment with 12-o-tetradecanoyl phorbol-13-acetate (TPA), a protein kinase C (PKC) activator, resulted in an increased secretion of PAcP in a dose- and time-dependent fashion. 4α-Phorbol, a biologically inactive isomer of TPA, had no effect. This TPA stimulation of PAcP secretion was observed 2 h after exposure, while TPA did not have a significant effect on the mRNA level even with a 6 h treatment. A23187 calcium ionophore, known to mobilize cellular calcium which is a co-factor of PKC, also activated PAcP secretion. This TPA stimulation of PAcP secretion was more potent than the conventional stimulating agent 5α-dihydrotestosterone (DHT) at the same concentration of 50 nM. Furthermore, the action of TPA and DHT on PAcP secretion was blocked by five different PKC inhibitors. Results also showed that DHT, as well as TPA, could rapidly modulate PKC activity. Therefore, PKC can regulate PAcP secretion, and may also be involved in DHT action on PAcP secretion.

Original languageEnglish (US)
Pages (from-to)1139-1148
Number of pages10
JournalCell Biology International
Volume25
Issue number11
DOIs
StatePublished - 2001

Keywords

  • Androgen regulation
  • Human prostate cancer cells
  • Prostatic acid phosphatase
  • Protein kinase C

ASJC Scopus subject areas

  • Cell Biology

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