Abstract
Forty-one rice cDNAs encoding protein kinases were fused to the tandem affinity purification (TAP) tag and expressed in transgenic rice plants. The TAP-tagged kinases and interacting proteins were purified from the T1 progeny of the transgenic rice plants and identified by mass spectrometry. Ninety-five percent of the TAP-tagged kinases were recovered. Fifty-six percent of the TAP-tagged kinases were found to interact with other rice proteins. A number of these interactions were consistent with known protein complexes found in other species, validating the TAP-tag method in rice plants. Phosphorylation sites were identified on four of the kinases that interacted with either 14-3-3 proteins or cyclins.
Original language | English (US) |
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Pages (from-to) | 1-13 |
Number of pages | 13 |
Journal | Plant Journal |
Volume | 46 |
Issue number | 1 |
DOIs | |
State | Published - Apr 2006 |
Keywords
- Affinity
- Oryza sativa
- Protein kinase
- Proteomics
- Tandem affinity purification
ASJC Scopus subject areas
- Genetics
- Plant Science
- Cell Biology