Protein-protein interactions of tandem affinity purified protein kinases from rice

Jai S. Rohila, Mei Chen, Shuo Chen, Johann Chen, Ronald L. Cerny, Christopher Dardick, Patrick Canlas, Hiroaki Fujii, Michael Gribskov, Siddhartha Kanrar, Lucas Knoflicek, Becky Stevenson, Mingtang Xie, Xia Xu, Xianwu Zheng, Jian Kang Zhu, Pamela Ronald, Michael E. Fromm

Research output: Contribution to journalArticle

30 Scopus citations

Abstract

Eighty-eight rice (Oryza sativa) cDNAs encoding rice leaf expressed protein kinases (PKs) were fused to a Tandem Affinity Purification tag (TAP-tag) and expressed in transgenic rice plants. The TAP-tagged PKs and interacting proteins were purified from the T1 progeny of the transgenic rice plants and identified by tandem mass spectrometry. Forty-five TAP-tagged PKs were recovered in this study and thirteen of these were found to interact with other rice proteins with a high probability score. In vivo phosphorylated sites were found for three of the PKs. A comparison of the TAP-tagged data from a combined analysis of 129 TAP-tagged rice protein kinases with a concurrent screen using yeast two hybrid methods identified an evolutionarily new rice protein that interacts with the well conserved cell division cycle 2 (CDC2) protein complex.

Original languageEnglish (US)
Article numbere6685
JournalPloS one
Volume4
Issue number8
DOIs
StatePublished - Aug 19 2009

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)
  • General

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    Rohila, J. S., Chen, M., Chen, S., Chen, J., Cerny, R. L., Dardick, C., Canlas, P., Fujii, H., Gribskov, M., Kanrar, S., Knoflicek, L., Stevenson, B., Xie, M., Xu, X., Zheng, X., Zhu, J. K., Ronald, P., & Fromm, M. E. (2009). Protein-protein interactions of tandem affinity purified protein kinases from rice. PloS one, 4(8), [e6685]. https://doi.org/10.1371/journal.pone.0006685