Proteomic analysis of atrazine exposure in Drosophila melanogaster (Diptera: Drosophilidae)

Benjamin J. Thornton, Thomas E. Elthon, Ronald L. Cerny, Blair D. Siegfried

Research output: Contribution to journalArticlepeer-review

25 Scopus citations

Abstract

Atrazine is a widely used herbicide that has been reported to induce the activity of certain detoxification enzymes and to affect insecticide toxicity in organisms experiencing simultaneous exposure to both atrazine and insecticides. In this study, the effects of atrazine (2-chloro-4-ethylamino-6-isopropylamino-1,3,5-triazine) exposure on protein expression in male and female Drosophila melanogaster adults in both microsomal and cytosolic cell fractions was investigated by 2-dimensional gel electrophoresis. Differentially expressed proteins (vs. controls) were identified using matrix assisted laser desorption-time (MALDI-TOF) of flight mass spectrometry (MS). We identified a total of 28 proteins associated with energy production including glycolysis and mitochondrial respiration as differentially expressed and nine proteins associated with detoxification and response to oxidative stress. Most of these proteins were expressed in one sex or the other but not in both. Surprisingly, the only proteins associated with detoxification were identified as glutathione transferases. No cytochrome P450s were identified which have previously been shown to be responsive to atrazine exposure in D. melanogaster and proposed to be associated with insecticide/atrazine interactions. Results of this investigation support the role of atrazine in affecting mitochondrial electron transport and oxidative stress. However, the role of atrazine in pesticide interactions remains uncertain.

Original languageEnglish (US)
Pages (from-to)235-241
Number of pages7
JournalChemosphere
Volume81
Issue number2
DOIs
StatePublished - Sep 2010

Keywords

  • Atrazine
  • Glutathione transferase
  • Mitochondrial electron transport
  • Pesticide interactions
  • Proteomics

ASJC Scopus subject areas

  • Environmental Engineering
  • Environmental Chemistry
  • Chemistry(all)
  • Pollution
  • Health, Toxicology and Mutagenesis

Fingerprint Dive into the research topics of 'Proteomic analysis of atrazine exposure in Drosophila melanogaster (Diptera: Drosophilidae)'. Together they form a unique fingerprint.

Cite this