Proteomic analysis of circulating monocytes in Chinese premenopausal females with extremely discordant bone mineral density

Fei Yan Deng, Yao Zhong Liu, Li Ming Li, Chen Jiang, Shan Wu, Yuan Chen, Hui Jiang, Fang Yang, Ji Xian Xiong, Peng Xiao, Su Mei Xiao, Li Jun Tan, Xiao Sun, Xue Zhen Zhu, Man Yuan Liu, Shu Feng Lei, Xiang Ding Chen, Jing Yun Xie, Gary G. Xiao, Song Ping LiangHong Wen Deng

Research output: Contribution to journalArticle

33 Scopus citations

Abstract

Osteoporosis (OP) is a major public health problem, mainly characterized by low bone mineral density (BMD). Circulating monocytes (CMCs) may serve as progenitors of osteoclasts and produce a wide variety of factors important to bone metabolism. However, the specific action mechanism of CMCs in the pathogenesis of OP is far from clear. We performed a comparative protein expression profiling study of CMCs in Chinese premenopausal females with extremely discordant BMD, identified a total of 38 differentially expressed proteins, and confirmed with Western blotting five proteins: ras suppressor protein1 (RSU1), gelsolin (GSN), manganesecontaining superoxide dismutase (SOD2), glutathione peroxidase 1(GPX1), and prolyl 4-hydroxylase b subunit (P4HB). These proteins might affect CMCs' trans-endothelium, differentiation, and/or downstream osteoclast functions, thus contribute to differential osteoclastogenesis and finally lead to BMD variation. The findings promote our understanding of the role of CMCs in BMD determination, and provide an insight into the pathogenesis of human OP.

Original languageEnglish (US)
Pages (from-to)4259-4272
Number of pages14
JournalProteomics
Volume8
Issue number20
DOIs
StatePublished - Oct 2008

Keywords

  • Bone mineral density
  • Circulating monocyte
  • Osteoclastogenesis
  • Protein

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

Fingerprint Dive into the research topics of 'Proteomic analysis of circulating monocytes in Chinese premenopausal females with extremely discordant bone mineral density'. Together they form a unique fingerprint.

  • Cite this

    Deng, F. Y., Liu, Y. Z., Li, L. M., Jiang, C., Wu, S., Chen, Y., Jiang, H., Yang, F., Xiong, J. X., Xiao, P., Xiao, S. M., Tan, L. J., Sun, X., Zhu, X. Z., Liu, M. Y., Lei, S. F., Chen, X. D., Xie, J. Y., Xiao, G. G., ... Deng, H. W. (2008). Proteomic analysis of circulating monocytes in Chinese premenopausal females with extremely discordant bone mineral density. Proteomics, 8(20), 4259-4272. https://doi.org/10.1002/pmic.200700480