TY - JOUR
T1 - Proteomic biosignatures for monocyte-macrophage differentiation
AU - Kraft-Terry, Stephanie D.
AU - Gendelman, Howard E.
N1 - Funding Information:
Grant support: Support for research was provided by National Institutes of Health Grants P20 DA026146 , 5P01 DA028555-02 , R01 NS36126 , P01 NS31492 , 2R01 NS034239 , P20 RR15635 , P01 MH64570 , and P01 NS43985 (to H.E.G).
PY - 2011
Y1 - 2011
N2 - We used pulsed stable isotope labeling of amino acids in cell culture (pSILAC) to assess protein dynamics during monocyte-macrophage differentiation. pSILAC allows metabolic labeling of newly synthesized proteins. Such de novo protein production was evaluated from 3 to 7 days in culture. Proteins were identified by liquid chromatography-tandem mass spectrometry then quantified by MaxQuant. Protein-protein linkages were then assessed by Ingenuity Pathway Analysis. Proteins identified were linked to cell homeostasis, free radical scavenging, molecular protein transport, carbohydrate metabolism, small molecule chemistry, and cell morphology. The data demonstrates specific biologic events that are linked to monocyte transformation in a defined biologic system.
AB - We used pulsed stable isotope labeling of amino acids in cell culture (pSILAC) to assess protein dynamics during monocyte-macrophage differentiation. pSILAC allows metabolic labeling of newly synthesized proteins. Such de novo protein production was evaluated from 3 to 7 days in culture. Proteins were identified by liquid chromatography-tandem mass spectrometry then quantified by MaxQuant. Protein-protein linkages were then assessed by Ingenuity Pathway Analysis. Proteins identified were linked to cell homeostasis, free radical scavenging, molecular protein transport, carbohydrate metabolism, small molecule chemistry, and cell morphology. The data demonstrates specific biologic events that are linked to monocyte transformation in a defined biologic system.
KW - Liquid chromatography-tandem mass spectrometry
KW - MaxQuant
KW - Monocyte differentiation
KW - Monocyte-derived macrophages
KW - Proteomic biosignatures
KW - Pulsed stable isotope labeling with amino acids in cell culture
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U2 - 10.1016/j.cellimm.2011.07.002
DO - 10.1016/j.cellimm.2011.07.002
M3 - Article
C2 - 21788015
AN - SCOPUS:80054041338
SN - 0008-8749
VL - 271
SP - 239
EP - 255
JO - Cellular Immunology
JF - Cellular Immunology
IS - 2
ER -