Proteomic Database Search and Analytical Quantification for Mass Spectrometry

M. Wojtkiewicz, J. Wiederin, P. Ciborowski

Research output: Chapter in Book/Report/Conference proceedingChapter

4 Scopus citations


Technological development and the ever-increasing complexity of proteomic profiling studies requires automated tools for tandem mass spectra interpretation. The analysis of various methods used for accurate and sensitive quantitation adds another layer of complexity. Therefore, investigators need a more in-depth understanding of database searches and the calculation of statistical significance in order to find a proper bioinformatics approach for their study. In this chapter, we review tools and procedures that have been developed in two decades of collective experience in the proteomics field. However, the field is still in dynamic expansion thus intention of this chapter is to provide readers with background for reading about the newest tools and approaches developed for database searches.

Original languageEnglish (US)
Title of host publicationProteomic Profiling and Analytical Chemistry
Subtitle of host publicationThe Crossroads: Second Edition
PublisherElsevier Inc.
Number of pages18
ISBN (Print)9780444636881
StatePublished - Mar 22 2016


  • Analytical quantification
  • Database search
  • Mass spectrometry data searches
  • Post-database search data processing
  • Protein databases
  • Search engines

ASJC Scopus subject areas

  • General Chemistry


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